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Flotillin 2 Polyclonal antibody, PBS Only

Flotillin 2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 28208-1-PBS

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Synonyms

验证数据展示

  • IHC staining of human breast cancer using 28208-1-AP (same clone as 28208-1-PBS)

    IHC staining of human breast cancer using 28208-1-AP (same clone as 28208-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28208-1-AP (Flotillin 2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28208-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FLOT2/Flotillin 2 antibody included
  • WB analysis using 28208-1-AP (same clone as 28208-1-PBS)

    WB analysis using 28208-1-AP (same clone as 28208-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28208-1-AP (Flotillin 2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28208-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 28208-1-AP (same clone as 28208-1-PBS)

    IF Staining of HeLa using 28208-1-AP (same clone as 28208-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Flotillin 2 antibody (28208-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 28208-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy FLOT2/Flotillin 2 Ready-To-Use IHC Kit. FLOT2/Flotillin 2 primary antibody included.

产品信息

28208-1-PBS targets Flotillin 2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen Flotillin 2 fusion protein Ag28308 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name flotillin 2
Synonyms
Calculated Molecular Weight 379 aa, 42 kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberBC017292
Gene Symbol Flotillin 2
Gene ID (NCBI) 2319
RRIDAB_2881089
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14254
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Flotillins are membrane-associated proteins that contain two homologous isoforms, flotillin 1 (FLOT1) and flotillin 2 (FLOT2). They are highly conserved and widely expressed proteins that commonly used as markers of lipid rafts. Flotillins are involved in various signaling pathways, cell adhesion, membrane trafficking and axonal growth.