• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Flotillin 2 Monoclonal antibody, PBS Only

Flotillin 2 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E11G12

Cat no : 66881-1-PBS

Print datasheet

Synonyms

FLOT2, Flotillin-2, ESA1, ESA, Epidermal surface antigen

验证数据展示

  • WB analysis using 66881-1-Ig (same clone as 66881-1-PBS)

    WB analysis using 66881-1-Ig (same clone as 66881-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66881-1-Ig (Flotillin 2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 66881-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66881-1-Ig (same clone as 66881-1-PBS)

    IHC staining of human breast cancer using 66881-1-Ig (same clone as 66881-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66881-1-Ig (Flotillin 2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66881-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FLOT2/Flotillin 2 antibody included
  • IHC staining of human breast cancer using 66881-1-Ig (same clone as 66881-1-PBS)

    IHC staining of human breast cancer using 66881-1-Ig (same clone as 66881-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66881-1-Ig (Flotillin 2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66881-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with FLOT2/Flotillin 2 antibody included
  • IF Staining of HeLa using 66881-1-Ig (same clone as 66881-1-PBS)

    IF Staining of HeLa using 66881-1-Ig (same clone as 66881-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Flotillin 2 antibody (66881-1-Ig, Clone: 1E11G12 ) at dilution of 1:4000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66881-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 66881-1-Ig (same clone as 66881-1-PBS)

    FC experiment of HeLa using 66881-1-Ig (same clone as 66881-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug Flotillin 2 Monoclonal antibody (66881-1-Ig, Clone:1E11G12) and CoraLite488-conjugated Donkey Anti-Mouse IgG(H+L)( SA00013-5)(red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66881-1-PBS in a different storage buffer formulation.

  • WB analysis using 66881-1-Ig (same clone as 66881-1-PBS)

    WB analysis using 66881-1-Ig (same clone as 66881-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66881-1-Ig (Flotillin 2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66881-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy FLOT2/Flotillin 2 Ready-To-Use IHC Kit. FLOT2/Flotillin 2 primary antibody included.

产品信息

66881-1-PBS targets Flotillin 2 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human, mouse, rat, pig, rabbit
Immunogen Flotillin 2 fusion protein Ag28308 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name flotillin 2
Synonyms FLOT2, Flotillin-2, ESA1, ESA, Epidermal surface antigen
Calculated Molecular Weight 379 aa, 42 kDa
Observed Molecular Weight 45 kDa
GenBank Accession NumberBC017292
Gene Symbol Flotillin 2
Gene ID (NCBI) 2319
RRIDAB_2882213
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ14254
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Flotillins are membrane-associated proteins that contain two homologous isoforms, flotillin 1 (FLOT1) and flotillin 2 (FLOT2). They are highly conserved and widely expressed proteins that commonly used as markers of lipid rafts. Flotillins are involved in various signaling pathways, cell adhesion, membrane trafficking and axonal growth.