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Flotillin 2 Monoclonal antibody, PBS Only

Flotillin 2 Monoclonal Antibody for WB,Indirect ELISA,IHC,IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig, Rabbit

Applications

WB,Indirect ELISA,IHC,IF

Conjugate

Unconjugated

CloneNo.

1E11G12

Cat No : 66881-1-PBS

Print datasheet

Synonyms

ECS 1, ECS1, Epidermal surface antigen, ESA, ESA1, FLOT2, flotillin 2, M17S1

验证数据展示

  • WB analysis using 66881-1-Ig (same clone as 66881-1-PBS)

    WB analysis using 66881-1-Ig (same clone as 66881-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66881-1-Ig (Flotillin 2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 66881-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66881-1-Ig (same clone as 66881-1-PBS)

    IHC staining of human breast cancer using 66881-1-Ig (same clone as 66881-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66881-1-Ig (Flotillin 2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66881-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66881-1-Ig (same clone as 66881-1-PBS)

    IHC staining of human breast cancer using 66881-1-Ig (same clone as 66881-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66881-1-Ig (Flotillin 2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66881-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66881-1-Ig (same clone as 66881-1-PBS)

    IF Staining of HeLa using 66881-1-Ig (same clone as 66881-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Flotillin 2 antibody (66881-1-Ig, Clone: 1E11G12 ) at dilution of 1:4000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66881-1-PBS in a different storage buffer formulation.

  • WB analysis using 66881-1-Ig (same clone as 66881-1-PBS)

    WB analysis using 66881-1-Ig (same clone as 66881-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66881-1-Ig (Flotillin 2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66881-1-PBS in a different storage buffer formulation.

产品信息

66881-1-PBS targets Flotillin 2 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Pig, Rabbit samples.

Tested Applications WB,Indirect ELISA,IHC,IF Application Description
Tested Reactivity Human, Mouse, Rat, Pig, Rabbit
Immunogen Flotillin 2 fusion protein Ag28308 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name flotillin 2
Synonyms ECS 1, ECS1, Epidermal surface antigen, ESA, ESA1, FLOT2, flotillin 2, M17S1
Calculated Molecular Weight 379 aa, 42 kDa
Observed Molecular Weight 45 kDa
GenBank Accession NumberBC017292
Gene Symbol FLOT2
Gene ID (NCBI) 2319
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ14254
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

Flotillins are membrane-associated proteins that contain two homologous isoforms, flotillin 1 (FLOT1) and flotillin 2 (FLOT2). They are highly conserved and widely expressed proteins that commonly used as markers of lipid rafts. Flotillins are involved in various signaling pathways, cell adhesion, membrane trafficking and axonal growth.