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验证数据展示

  • WB analysis using 60060-1-Ig (same clone as 60060-1-PBS)

    WB analysis using 60060-1-Ig (same clone as 60060-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 60060-1-Ig (Follistatin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60060-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary tumor using 60060-1-Ig (same clone as 60060-1-PBS)

    IHC staining of human ovary tumor using 60060-1-Ig (same clone as 60060-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60060-1-Ig (Follistatin antibody at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60060-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary tumor using 60060-1-Ig (same clone as 60060-1-PBS)

    IHC staining of human ovary tumor using 60060-1-Ig (same clone as 60060-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60060-1-Ig (Follistatin antibody at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60060-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 60060-1-Ig (same clone as 60060-1-PBS)

    IF Staining of HeLa using 60060-1-Ig (same clone as 60060-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Follistatin antibody (60060-1-Ig, Clone: 4E9B10 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60060-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

Follistatin Monoclonal antibody, PBS Only

Follistatin Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 60060-1-PBS

产品说明书

CloneNo. 4E9B10

宿主/亚型

Mouse / IgG2b

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

FST, FS, Activin-binding protein, Activin binding protein, 4E9B10

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

60060-1-PBS targets Follistatin in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原 Follistatin fusion protein Ag0775 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 follistatin
别名 FST, FS, Activin-binding protein, Activin binding protein, 4E9B10
计算分子量 33 aa, 7 kDa
观测分子量 35 kDa
GenBank蛋白编号BC004107
基因名称 Follistatin
Gene ID (NCBI) 10468
RRIDAB_2106706
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP19883
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Follistatin (FST) is a member of the tissue growth factor β family and is a secreted glycoprotein that antagonizes many members of the family, including activin A, growth differentiation factor11 , and myostatin. It binds activin A with high affinity and whose expression can be induced by activin A and several other pro-inflammatory cytokines. Activin A-follistatin complexes are biologically inactive and bind to cell surface heparan sulphate-containing proteoglycans for internalisation and degradation. It has also been found to play a significant role in the management of skeletal muscle size and mass. It also has important roles in early embryonic development, differentiation of ovarian granulosa cells, liver fibrosis and polycystic ovarian syndrome. FS 288 glycosylation results in a major species of 35 kDa and a less abundant 40 kDa and minor products of 40 and 42 kDa (PMID: 9785474). Three typical follistatin bands are at 32, 35, and 39 kDa (PMID: 2036994).