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  • Featured Product
  • KD/KO Validated

Follistatin Monoclonal antibody, PBS Only

Follistatin Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4E9B10

Cat no : 60060-1-PBS

Print datasheet

Synonyms

Activin binding protein, follistatin, FS, FST

验证数据展示

  • WB analysis using 60060-1-Ig (same clone as 60060-1-PBS)

    WB analysis using 60060-1-Ig (same clone as 60060-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 60060-1-Ig (Follistatin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60060-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary tumor using 60060-1-Ig (same clone as 60060-1-PBS)

    IHC staining of human ovary tumor using 60060-1-Ig (same clone as 60060-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60060-1-Ig (Follistatin antibody at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60060-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary tumor using 60060-1-Ig (same clone as 60060-1-PBS)

    IHC staining of human ovary tumor using 60060-1-Ig (same clone as 60060-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 60060-1-Ig (Follistatin antibody at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60060-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 60060-1-Ig (same clone as 60060-1-PBS)

    IF Staining of HeLa using 60060-1-Ig (same clone as 60060-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Follistatin antibody (60060-1-Ig, Clone: 4E9B10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60060-1-PBS in a different storage buffer formulation.

产品信息

60060-1-PBS targets Follistatin in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen Follistatin fusion protein Ag0775 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name follistatin
Synonyms Activin binding protein, follistatin, FS, FST
Calculated Molecular Weight 33 aa, 7 kDa
Observed Molecular Weight 35 kDa
GenBank Accession NumberBC004107
Gene Symbol Follistatin
Gene ID (NCBI) 10468
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP19883
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Follistatin (FST) is a member of the tissue growth factor β family and is a secreted glycoprotein that antagonizes many members of the family, including activin A, growth differentiation factor11 , and myostatin. It binds activin A with high affinity and whose expression can be induced by activin A and several other pro-inflammatory cytokines. Activin A-follistatin complexes are biologically inactive and bind to cell surface heparan sulphate-containing proteoglycans for internalisation and degradation. It has also been found to play a significant role in the management of skeletal muscle size and mass. It also has important roles in early embryonic development, differentiation of ovarian granulosa cells, liver fibrosis and polycystic ovarian syndrome. FS 288 glycosylation results in a major species of 35 kDa and a less abundant 40 kDa and minor products of 40 and 42 kDa (PMID: 9785474). Three typical follistatin bands are at 32, 35, and 39 kDa (PMID: 2036994).