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G-CSF Polyclonal antibody

G-CSF Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 30415-1-AP

Print datasheet

Synonyms

Csf3, Granulocyte colony-stimulating factor, G CSF, Csfg

验证数据展示

  • WB analysis using 30415-1-AP

    WB analysis using 30415-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30415-1-AP (G-CSF antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, K-562 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30415-1-AP targets G-CSF in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name colony stimulating factor 3 (granulocyte)
Synonyms Csf3, Granulocyte colony-stimulating factor, G CSF, Csfg
Calculated Molecular Weight22kd
Observed Molecular Weight18-20 kDa
GenBank Accession NumberNM_009971
Gene Symbol Csf3
Gene ID (NCBI) 12985
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP09920
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Granulocyte colony-stimulating factor (G-CSF), also referred to as CSF3, is a protective cytokine with anti-inflammatory effects. G-CSF is important in promoting survival of the granulocytic lineage cells and proliferation and migration of neutrophils as well as trophoblast cells. G-CSF acts by binding to its receptor G-CSFR (also called CSF3R), which after binding with G-CSF activates the canonical Janus kinase (Jak)/signal transducer, activator of transcription (STAT)and Ras/Raf/MAP kinase pathways. G-CSF potently stimulates the proliferation and release of peripheral blood progenitor cells into the bloodstream and is therefore used to treat neutropenia after chemotherapy. Furthermore, G-CSF levels are elevated upon intensive exercise leading to increased neutrophil counts, which are predominantly due to delayed neutrophil apoptosis.

实验方案

Product Specific Protocols
WB protocol for G-CSF antibody 30415-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols