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验证数据展示

  • WB analysis using 66486-1-Ig (same clone as 66486-1-PBS)

    WB analysis using 66486-1-Ig (same clone as 66486-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66486-1-Ig (G3BP1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66486-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66486-1-Ig (same clone as 66486-1-PBS)

    IF Staining of HeLa using 66486-1-Ig (same clone as 66486-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed sodium arsenite treated HeLa cells using G3BP1 antibody (66486-1-Ig, Clone: 1E4A2 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66486-1-PBS in a different storage buffer formulation.

  • IHC staining of human testis using 66486-1-Ig (same clone as 66486-1-PBS)

    IHC staining of human testis using 66486-1-Ig (same clone as 66486-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66486-1-Ig (G3BP antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66486-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with G3BP1 antibody included
  • IHC staining of human testis using 66486-1-Ig (same clone as 66486-1-PBS)

    IHC staining of human testis using 66486-1-Ig (same clone as 66486-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66486-1-Ig (G3BP antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66486-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with G3BP1 antibody included
  • Featured Product
  • KD/KO验证

G3BP1 Monoclonal antibody, PBS Only

G3BP1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66486-1-PBS

产品说明书

CloneNo. 1E4A2

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat, pig

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

G3BP, G3BP 1, G3BP1, HDH VIII

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy G3BP1 Ready-To-Use IHC Kit. G3BP1 primary antibody included.

产品信息

66486-1-PBS targets G3BP1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 Human, mouse, rat, pig
免疫原 G3BP1 fusion protein Ag3728 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 GTPase activating protein (SH3 domain) binding protein 1
别名 G3BP, G3BP 1, G3BP1, HDH VIII
计算分子量 466 aa, 52 kDa
观测分子量 68 kDa
GenBank蛋白编号BC006997
基因名称 G3BP1
Gene ID (NCBI) 10146
RRIDAB_2819031
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ13283
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GAP SH3 Binding Protein 1 (G3BP1), also named as G3BP, is an effector of stress granule (SG) assembly. SG biology plays an important role in the pathophysiology of TDP-43 in ALS and FTLD-U. G3BP1 can be used as a marker of SG. It has been shown to function downstream of Ras and play a role in RNA metabolism, signal transduction, and proliferation. G3BP1 is a ubiquitously expressed protein that localizes to the cytoplasm in proliferating cells and to the nucleus in non-proliferating cells. G3BP1 has recently been implicated in cancer biology.