• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

G3BP1 Monoclonal antibody, PBS Only

G3BP1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E4A2

Cat no : 66486-1-PBS

Print datasheet

Synonyms

G3BP, G3BP 1, G3BP1, HDH VIII

验证数据展示

  • WB analysis using 66486-1-Ig (same clone as 66486-1-PBS)

    WB analysis using 66486-1-Ig (same clone as 66486-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66486-1-Ig (G3BP1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66486-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66486-1-Ig (same clone as 66486-1-PBS)

    IF Staining of HeLa using 66486-1-Ig (same clone as 66486-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed sodium arsenite treated HeLa cells using G3BP1 antibody (66486-1-Ig, Clone: 1E4A2 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66486-1-PBS in a different storage buffer formulation.

  • IHC staining of human testis using 66486-1-Ig (same clone as 66486-1-PBS)

    IHC staining of human testis using 66486-1-Ig (same clone as 66486-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66486-1-Ig (G3BP antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66486-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with G3BP1 antibody included
  • IHC staining of human testis using 66486-1-Ig (same clone as 66486-1-PBS)

    IHC staining of human testis using 66486-1-Ig (same clone as 66486-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66486-1-Ig (G3BP antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66486-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with G3BP1 antibody included
Planning an IHC experiment? We recommend our IHCeasy G3BP1 Ready-To-Use IHC Kit. G3BP1 primary antibody included.

产品信息

66486-1-PBS targets G3BP1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human, mouse, rat, pig
Immunogen G3BP1 fusion protein Ag3728 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name GTPase activating protein (SH3 domain) binding protein 1
Synonyms G3BP, G3BP 1, G3BP1, HDH VIII
Calculated Molecular Weight 466 aa, 52 kDa
Observed Molecular Weight 68 kDa
GenBank Accession NumberBC006997
Gene Symbol G3BP1
Gene ID (NCBI) 10146
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13283
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GAP SH3 Binding Protein 1 (G3BP1), also named as G3BP, is an effector of stress granule (SG) assembly. SG biology plays an important role in the pathophysiology of TDP-43 in ALS and FTLD-U. G3BP1 can be used as a marker of SG. It has been shown to function downstream of Ras and play a role in RNA metabolism, signal transduction, and proliferation. G3BP1 is a ubiquitously expressed protein that localizes to the cytoplasm in proliferating cells and to the nucleus in non-proliferating cells. G3BP1 has recently been implicated in cancer biology.