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G3BP2 Monoclonal antibody

G3BP2 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68580-1-Ig

产品说明书

CloneNo. 2E5G3

宿主/亚型

Mouse / IgG1

种属反应性

human, rat

应用

WB, IF/ICC, ELISA

G3BP 2, 2E5G3, Ras GTPase-activating protein-binding protein 2, GAP SH3 domain-binding protein 2, GAP SH3 domain binding protein 2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHeLa cells, A549 cells, HCT 116 cells, HepG2 cells, rat brain tissue, SCaBER cells, K-562 cells, MOLT-4 cells, HT-29 cells
Positive IF/ICC detected insodium arsenite treated HeLa cells
Planning an IHC experiment? We recommend our IHCeasy G3BP2 Ready-To-Use IHC Kit. G3BP2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68580-1-Ig targets G3BP2 in WB, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, rat
免疫原 G3BP2 fusion protein Ag9222 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 GTPase activating protein (SH3 domain) binding protein 2
别名 G3BP 2, 2E5G3, Ras GTPase-activating protein-binding protein 2, GAP SH3 domain-binding protein 2, GAP SH3 domain binding protein 2
计算分子量482aa,54 kDa; 449aa,51 kDa
观测分子量54 kDa
GenBank蛋白编号BC011731
基因名称 G3BP2
Gene ID (NCBI) 9908
RRIDAB_3085280
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9UN86
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Stress granules (SGs) are cytoplasmic mRNA-protein condensates formed in response to cellular stressors, such as oxidative stress, ultraviolet radiation, and viral infection (1). The Ras-GTPase-activating protein-binding proteins (G3BPs), consisting of G3BP1 and G3BP2, are key nucleating factors essential for SG formation. They function to protect RNAs from harmful conditions. G3BP2 is mainly distributed in the cytoplasm and participates in the formation of stress granules, cell differentiation, proliferation, and signal transduction. Accumulating evidence has demonstrated that aberrant expression of G3BP2 contributes to cancer initiation and progression, such as high expression of G3BP2 increasing cell stemness, metastasis and chemoresistance in breast cancer.

实验方案

Product Specific Protocols
WB protocol for G3BP2 antibody 68580-1-IgDownload protocol
IF protocol for G3BP2 antibody 68580-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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