GAA Polyclonal antibody

GAA Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 14367-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (2)

应用

WB, IP, IHC, ELISA and More (1)

Acid maltase, Aglucosidase alfa, GAA, glucosidase, alpha; acid, LYAG, Lysosomal alpha glucosidase

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inDU 145 cells, mouse liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman pancreas cancer tissue, mouse liver tissue, mouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy GAA Ready-To-Use IHC Kit. GAA primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14367-1-AP targets GAA in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IP, IHC, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse
文献引用反应性human, mouse, rat, zebrafish
免疫原 GAA fusion protein Ag5690 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 glucosidase, alpha; acid
别名 Acid maltase, Aglucosidase alfa, GAA, glucosidase, alpha; acid, LYAG, Lysosomal alpha glucosidase
计算分子量 105 kDa
观测分子量70-76 kDa
GenBank蛋白编号BC040431
基因名称 GAA
Gene ID (NCBI) 2548
RRIDAB_2107667
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP10253
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GAA(Alpha-1,4-glucosidase) is a lysosomal enzyme involved in the degradation of glycogen within cellular vacuoles. After translation, GAA undergoes proteolytic processing to form two lengths of lysosomal a-glucosidase, and both N-terminal and C-terminal processing occur. The full sequence can be further processed into a mature form. The 105 kDa GAA can be cleaved into 70 kDa or 76 kDa form.

实验方案

Product Specific Protocols
WB protocol for GAA antibody 14367-1-APDownload protocol
IHC protocol for GAA antibody 14367-1-APDownload protocol
IP protocol for GAA antibody 14367-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Nat Commun

Spatial metabolomics reveals glycogen as an actionable target for pulmonary fibrosis

Authors - Lindsey R Conroy
human,mouseWB,IF

Redox Biol

Dysfunction of astrocytic glycophagy exacerbates reperfusion injury in ischemic stroke

Authors - Haiyun Guo
humanIF

J Med Chem

5-C-Branched Deoxynojirimycin: Strategy for Designing a 1-Deoxynojirimycin-Based Pharmacological Chaperone with a Nanomolar Affinity for Pompe Disease.

Authors - Atsushi Kato
humanIF

Org Biomol Chem

Introduction of C-alkyl branches to L-iminosugars changes their active site binding orientation

Authors - Atsushi Kato
zebrafishWB

DNA Cell Biol

Disruption of the gaa Gene in Zebrafish Fails to Generate the Phenotype of Classical Pompe Disease.

Authors - Jing Wu
mouseWB

Curr Res Physiol

Glycophagy is involved in cardiac glycogen regulation in response to exercise

Authors - Samuel L James
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