GAA Polyclonal antibody

GAA Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IP, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 14367-1-AP

Print datasheet

Synonyms

Acid maltase, Aglucosidase alfa, GAA, glucosidase, alpha; acid, LYAG, Lysosomal alpha glucosidase



经过测试的应用

Positive WB detected inDU 145 cells, mouse liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman pancreas cancer tissue, mouse liver tissue, mouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy GAA Ready-To-Use IHC Kit. GAA primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14367-1-AP targets GAA in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat, zebrafish
Immunogen GAA fusion protein Ag5690 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name glucosidase, alpha; acid
Synonyms Acid maltase, Aglucosidase alfa, GAA, glucosidase, alpha; acid, LYAG, Lysosomal alpha glucosidase
Calculated Molecular Weight 105 kDa
Observed Molecular Weight70-76 kDa
GenBank Accession NumberBC040431
Gene Symbol GAA
Gene ID (NCBI) 2548
RRIDAB_2107667
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP10253
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GAA(Alpha-1,4-glucosidase) is a lysosomal enzyme involved in the degradation of glycogen within cellular vacuoles. After translation, GAA undergoes proteolytic processing to form two lengths of lysosomal a-glucosidase, and both N-terminal and C-terminal processing occur. The full sequence can be further processed into a mature form. The 105 kDa GAA can be cleaved into 70 kDa or 76 kDa form.

实验方案

Product Specific Protocols
WB protocol for GAA antibody 14367-1-APDownload protocol
IHC protocol for GAA antibody 14367-1-APDownload protocol
IP protocol for GAA antibody 14367-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Nat Commun

Spatial metabolomics reveals glycogen as an actionable target for pulmonary fibrosis

Authors - Lindsey R Conroy
human,mouseWB,IF

Redox Biol

Dysfunction of astrocytic glycophagy exacerbates reperfusion injury in ischemic stroke

Authors - Haiyun Guo
humanIF

J Med Chem

5-C-Branched Deoxynojirimycin: Strategy for Designing a 1-Deoxynojirimycin-Based Pharmacological Chaperone with a Nanomolar Affinity for Pompe Disease.

Authors - Atsushi Kato
humanIF

Org Biomol Chem

Introduction of C-alkyl branches to L-iminosugars changes their active site binding orientation

Authors - Atsushi Kato
zebrafishWB

DNA Cell Biol

Disruption of the gaa Gene in Zebrafish Fails to Generate the Phenotype of Classical Pompe Disease.

Authors - Jing Wu
mouseWB

Curr Res Physiol

Glycophagy is involved in cardiac glycogen regulation in response to exercise

Authors - Samuel L James