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GAB1 Polyclonal antibody

GAB1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 26200-1-AP

Print datasheet

Synonyms

Growth factor receptor bound protein 2-associated protein 1, GRB2-associated-binding protein 1, GRB2-associated binder 1, GRB2 associated binder 1

验证数据展示

  • WB analysis using 26200-1-AP

    WB analysis using 26200-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26200-1-AP (GAB1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 26200-1-AP

    IHC staining of mouse brain using 26200-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26200-1-AP (GAB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 26200-1-AP

    IHC staining of mouse brain using 26200-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26200-1-AP (GAB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHEK-293 cells, K-562 cells, C6 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26200-1-AP targets GAB1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen GAB1 fusion protein Ag24395 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name GRB2-associated binding protein 1
Synonyms Growth factor receptor bound protein 2-associated protein 1, GRB2-associated-binding protein 1, GRB2-associated binder 1, GRB2 associated binder 1
Calculated Molecular Weight 724 aa, 80 kDa
Observed Molecular Weight110 kDa
GenBank Accession NumberBC064848
Gene Symbol GAB1
Gene ID (NCBI) 2549
RRIDAB_2918101
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13480
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GAB1 antibody 26200-1-APDownload protocol
IHC protocol for GAB1 antibody 26200-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Anal Chem

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Authors - Hongwei Chu
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humanWB,IF

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Authors - Nai-Jie Fu
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