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  • Featured Product
  • KD/KO Validated

GAB2 Polyclonal antibody, PBS Only

GAB2 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 29272-1-PBS

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Synonyms

验证数据展示

  • WB analysis using 29272-1-AP (same clone as 29272-1-PBS)

    WB analysis using 29272-1-AP (same clone as 29272-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 29272-1-AP (GAB2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29272-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 29272-1-AP (same clone as 29272-1-PBS)

    IHC staining of human colon cancer using 29272-1-AP (same clone as 29272-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29272-1-AP (GAB2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29272-1-PBS in a different storage buffer formulation.

  • WB analysis of K-562 using 29272-1-AP (same clone as 29272-1-PBS)

    WB analysis of K-562 using 29272-1-AP (same clone as 29272-1-PBS)

    WB result of GAB2 antibody (29272-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GAB2 transfected K-562 cells. This data was developed using the same antibody clone with 29272-1-PBS in a different storage buffer formulation.

产品信息

29272-1-PBS targets GAB2 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen GAB2 fusion protein Ag30347 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name GRB2-associated binding protein 2
Synonyms
Calculated Molecular Weight 676 aa, 74 kDa
Observed Molecular Weight 74-90 kDa
GenBank Accession NumberBC131711
Gene Symbol GAB2
Gene ID (NCBI) 9846
RRIDAB_2918265
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UQC2
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Growth factor receptor-bound protein 2 (GAB2), an adaptor protein, belongs to the Gab family and is involved in various biologic processes. Gab2 mainly mediates phosphatidylinositol 3-kinase (PI3K) and extracellular signal-regulated kinase (ERK) signaling pathways. Furthermore, Gab2 governs tumorigenic signaling and is a novel potential therapeutic target in leukemia, breast cancer, ovarian cancer, and melanoma. Gab2 is the major 98-kDa phosphoprotein associated with SHP-2, PI 3-kinase, and Grb2 in normal human lymphocytes. (PMID: 32269703, PMID: 28842424, PMID: 28420432, PMID: 10849428)