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验证数据展示

  • WB analysis of mouse brain using 20089-1-AP

    WB analysis of mouse brain using 20089-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 20089-1-AP (GAB3-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of MCF-7 using 20089-1-AP

    IP experiment of MCF-7 using 20089-1-AP

    IP result of anti-GAB3-Specific (IP:20089-1-AP, 4ug; Detection:20089-1-AP 1:2000) with MCF-7 cells lysate 3200ug.

  • IHC staining of human liver cancer using 20089-1-AP

    IHC staining of human liver cancer using 20089-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20089-1-AP (GAB3-Specific Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human liver cancer using 20089-1-AP

    IHC staining of human liver cancer using 20089-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20089-1-AP (GAB3-Specific Antibody) at dilution of 1:200 (under 40x lens).

  • IF Staining of MCF-7 using 20089-1-AP

    IF Staining of MCF-7 using 20089-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 20089-1-AP (GAB3-Specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

GAB3-Specific Polyclonal antibody

GAB3-Specific Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 20089-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA

别名

GAB3, Growth factor receptor bound protein 2-associated protein 3, GRB2-associated-binding protein 3, GRB2-associated binder 3, GRB2 associated binder 3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, HepG2 cells, MCF-7 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20089-1-AP targets GAB3-Specific in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 GRB2-associated binding protein 3
别名 GAB3, Growth factor receptor bound protein 2-associated protein 3, GRB2-associated-binding protein 3, GRB2-associated binder 3, GRB2 associated binder 3
计算分子量 66 kDa
观测分子量 60-66 kDa
GenBank蛋白编号NM_001081573
基因名称 GAB3
Gene ID (NCBI) 139716
RRIDAB_11182715
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8WWW8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GAB3 belongs to the GAB family. GAB3 interacts with PIK3R/p85, SHP2 and GRAP2/MONA. It may interact with Grb2. The antibody is specific to GAB3.

实验方案

Product Specific Protocols
WB protocol for GAB3-Specific antibody 20089-1-APDownload protocol
IHC protocol for GAB3-Specific antibody 20089-1-APDownload protocol
IF protocol for GAB3-Specific antibody 20089-1-APDownload protocol
IP protocol for GAB3-Specific antibody 20089-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols