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GABPA Monoclonal antibody, PBS Only

GABPA Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2A7E3

Cat no : 68645-1-PBS

Print datasheet

Synonyms

GABP subunit alpha, GA-binding protein alpha chain, GA binding protein alpha chain, E4TF1A, E4TF1 60

验证数据展示

  • WB analysis using 68645-1-Ig (same clone as 68645-1-PBS)

    WB analysis using 68645-1-Ig (same clone as 68645-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68645-1-Ig (GABPA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68645-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 68645-1-Ig (same clone as 68645-1-PBS)

    IF Staining of HEK-293 using 68645-1-Ig (same clone as 68645-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using GABPA antibody (68645-1-Ig, Clone: 2A7E3 ) at dilution of 1:2000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-phalloidin (red). This data was developed using the same antibody clone with 68645-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy GABPA Ready-To-Use IHC Kit. GABPA primary antibody included.

产品信息

68645-1-PBS targets GABPA in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen GABPA fusion protein Ag33705 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name GA binding protein transcription factor, alpha subunit 60kDa
Synonyms GABP subunit alpha, GA-binding protein alpha chain, GA binding protein alpha chain, E4TF1A, E4TF1 60
Calculated Molecular Weight 454 aa, 51 kDa
Observed Molecular Weight 56-60 kDa
GenBank Accession NumberBC035031
Gene Symbol GABPA
Gene ID (NCBI) 2551
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ06546
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GA-binding protein alpha chain (GABP alpha subunit, GABPA, nuclear respiratory factor 2 subunit alpha, transcription factor E4TF1-60) is one of three GA-binding protein transcription factor subunits which functions as a DNA-binding subunit.GABPA is a member of Ets family, binds to the Yap promoter and activates YAP transcription(23684612). Since this subunit shares identity with a subunit encoding the nuclear respiratory factor 2 gene, it is likely involved in activation of cytochrome oxidase expression and nuclear control of mitochondrial function. This subunit also shares identity with a subunit constituting the transcription factor E4TF1, responsible for expression of the adenovirus E4 gene. Because of its chromosomal localization and ability to form heterodimers with other polypeptides, this gene may play a role in the Down Syndrome phenotype.