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GABRA2 Recombinant antibody

GABRA2 Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230158E6

Cat No : 83057-2-RR

Print datasheet

Synonyms

230158E6

验证数据展示

  • WB analysis using 83057-2-RR

    WB analysis using 83057-2-RR

    mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 83057-2-RR (GABRA2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis using 83057-2-RR

    WB analysis using 83057-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83057-2-RR (GABRA2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using 83057-2-RR

    FC experiment of HeLa using 83057-2-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug GABRA2 Recombinant antibody (83057-2-RR, Clone:230158E6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83057-2-RR

    Affinity and Kinetic Characterization of 83057-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83057-2-RR against Human GABRA2 were performed. The affinity constant is 0.403 nM.

经过测试的应用

Positive WB detected inmouse cerebellum tissue, mouse brain tissue, rat brain tissue
Positive FC (Intra) detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83057-2-RR targets GABRA2 in WB, FC (Intra), ELISA applications and shows reactivity with mouse, rat samples.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity mouse, rat
Immunogen GABRA2 fusion protein Ag29301 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name gamma-aminobutyric acid (GABA) A receptor, alpha 2
Synonyms 230158E6
Calculated Molecular Weight 51 kDa
Observed Molecular Weight50-51 kDa
GenBank Accession NumberNM_000807
Gene Symbol GABRA2
Gene ID (NCBI) 2555
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP47869
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GABRA2 (gamma-aminobutyric acid type A receptor subunit alpha2), also known as DEE78. It is expected to be located in cell membrane, and the protein is highly expressed in brain. The protein is a ligand-gated chloride channel, which is a component of the heteropentameric receptor for GABA, the major inhibitory neurotransmitter in the brain (PubMed:29961870). It also plays an important role in the formation of functional inhibitory GABAergic synapses in addition to mediating synaptic inhibition as a GABA-gated ion channel (PubMed:29961870). The calculated molecular weight of GABRA2 is 51 kDa.

实验方案

Product Specific Protocols
WB protocol for GABRA2 antibody 83057-2-RRDownload protocol
FC protocol for GABRA2 antibody 83057-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols