验证数据展示
经过测试的应用
| Positive WB detected in | Neuro-2a cells, mouse brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IF-P detected in | mouse cerebellum tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14104-1-AP targets GABRG2 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF-P, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | GABRG2 fusion protein Ag5237 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | gamma-aminobutyric acid (GABA) A receptor, gamma 2 |
| 别名 | Gamma-aminobutyric acid receptor subunit gamma-2, GABAAR subunit gamma-2, GABA(A) receptor subunit gamma-2, GABA A receptor gamma 2, ECA2 |
| 计算分子量 | 467 aa, 54 kDa |
| 观测分子量 | 54-60 kDa |
| GenBank蛋白编号 | BC059389 |
| 基因名称 | GABRG2 |
| Gene ID (NCBI) | 2566 |
| RRID | AB_10693527 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P18507 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GABRG2 (gamma-aminobutyric acid type A receptor subunit gamma2), which is expected to be located in cell membrane and expressed in brain and retina. The calculated molecular weight of the protein is 54 kDa. This antibody can recognize the 43 kDa band of target (PMID:12091434). And it also has glycosylation modification. GABA is the major inhibitory neurotransmitter in the mammlian brain, where it acts at GABA-A receptors, which are ligand-gated chloride channels. GABA-A receptors are pentameric, consisting of proteins from several subunit classes: alpha, beta, gamma, delta and rho. Mutations in this gene have been associated with epilepsy and febrile seizures.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GABRG2 antibody 14104-1-AP | Download protocol |
| IF protocol for GABRG2 antibody 14104-1-AP | Download protocol |
| IP protocol for GABRG2 antibody 14104-1-AP | Download protocol |
| FC protocol for GABRG2 antibody 14104-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
CNS Neurosci Ther STK24 modulates excitatory synaptic transmission in epileptic hippocampal neurons. | ||
Neurosci Lett Acupuncture Improves Locomotor Function by Enhancing GABA receptor Expression in Transient Focal Cerebral Ischemia Rats. | ||
Neuropharmacology A role of GABAA receptor α1 subunit in the hippocampus for rapid-acting antidepressant-like effects of ketamine | ||
Front Endocrinol (Lausanne) Altered GnRH neuron and ovarian innervation characterize reproductive dysfunction linked to the Fragile X messenger ribonucleoprotein (Fmr1) gene mutation | ||
Nat Genet Pan-cancer analysis identifies tumor-specific antigens derived from transposable elements |