验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, A549 cells, HeLa cells |
Positive IP detected in | mouse kidney tissue |
Positive IHC detected in | human heart tissue, human kidney tissue, human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HEK-293 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
19958-1-AP targets GAC-specific in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.
经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
文献引用应用 | WB, IHC, IF, IP, CoIP |
经测试反应性 | human, mouse |
文献引用反应性 | human, mouse, rat |
免疫原 | Peptide 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | glutaminase |
别名 | GLS, KGA, GLS1, GAM, GAC |
计算分子量 | 65 kDa |
观测分子量 | 58 kDa |
GenBank蛋白编号 | AF158555 |
基因名称 | GLS |
Gene ID (NCBI) | 2744 |
RRID | AB_10640899 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | O94925 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Glutaminase C (GAC), a splicing variant of the kidney-type glutaminase (KGA) gene, is a vital mitochondrial enzyme protein that catalyzes glutamine to glutamate. GAC is the only isoform present in the mitochondria (PMID:22228304). In a normal state, immunodecorationwith antibodies directed against GAC identified an abundant complex of 232 kDa as a tetrameric form of GAC, the DPAA can directly destructed the native tetrameric GAC complex to accumulate the unassembled one (50-90 kDa). (PMID:22493432). This antibody is specific to GAC isoform. It has no cross reaction to KGA.
实验方案
Product Specific Protocols | |
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WB protocol for GAC-specific antibody 19958-1-AP | Download protocol |
IHC protocol for GAC-specific antibody 19958-1-AP | Download protocol |
IF protocol for GAC-specific antibody 19958-1-AP | Download protocol |
IP protocol for GAC-specific antibody 19958-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cell Metab Memory CD8+ T Cells Balance Pro- and Anti-inflammatory Activity by Reprogramming Cellular Acetate Handling at Sites of Infection. | ||
Cell Stem Cell Differential Alternative Polyadenylation Landscapes Mediate Hematopoietic Stem Cell Activation and Regulate Glutamine Metabolism. | ||
Cancer Commun (Lond) Glutamine metabolic microenvironment drives M2 macrophage polarization to mediate trastuzumab resistance in HER2-positive gastric cancer | ||
Blood Targeting glutaminolysis has anti-leukemic activity in acute myeloid leukemia and synergizes with BCL-2 inhibition. | ||
Elife Metabolic reprogramming of cancer cells by JMJD6-mediated pre-mRNA splicing associated with therapeutic response to splicing inhibitor | ||
Oncotarget Discovery of selective inhibitors of Glutaminase-2, which inhibit mTORC1, activate autophagy and inhibit proliferation in cancer cells. |