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GAD1 Monoclonal antibody, PBS Only

GAD1 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2D5B7

Cat no : 67648-1-PBS

Print datasheet

Synonyms

FLJ45882, GAD, GAD 67, GAD1, GAD67, Glutamate decarboxylase 1, SCP

验证数据展示

  • WB analysis using 67648-1-Ig (same clone as 67648-1-PBS)

    WB analysis using 67648-1-Ig (same clone as 67648-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67648-1-Ig (GAD1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67648-1-PBS in a different storage buffer formulation.

  • IHC staining of rat cerebellum using 67648-1-Ig (same clone as 67648-1-PBS)

    IHC staining of rat cerebellum using 67648-1-Ig (same clone as 67648-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 67648-1-Ig (GAD1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67648-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GAD1 antibody included
  • IHC staining of mouse cerebellum using 67648-1-Ig (same clone as 67648-1-PBS)

    IHC staining of mouse cerebellum using 67648-1-Ig (same clone as 67648-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67648-1-Ig (GAD1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67648-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GAD1 antibody included
  • IF Staining of rat brain using 67648-1-Ig (same clone as 67648-1-PBS)

    IF Staining of rat brain using 67648-1-Ig (same clone as 67648-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using GAD1 antibody (67648-1-Ig, Clone: 2D5B7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67648-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy GAD1 Ready-To-Use IHC Kit. GAD1 primary antibody included.

产品信息

67648-1-PBS targets GAD1 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.

Tested Applications WB, IHC, IF-P, Indirect ELISA Application Description
Tested Reactivity Human, mouse, rat, pig, rabbit
Immunogen GAD1 fusion protein Ag21336 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name glutamate decarboxylase 1 (brain, 67kDa)
Synonyms FLJ45882, GAD, GAD 67, GAD1, GAD67, Glutamate decarboxylase 1, SCP
Calculated Molecular Weight 67 kDa
Observed Molecular Weight 67 kDa, 57 kDa
GenBank Accession NumberBC002815
Gene Symbol GAD1
Gene ID (NCBI) 2571
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ99259
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GAD1, also named as GAD67, belongs to the group II decarboxylase family. GAD1 is the rate-limiting enzyme responsible for γ-aminobutyric acid (GABA) biosynthesis from glutamatic acid and the major GAD isoform in the human brain for early brain development (PMID:21302352). It is also potentially involved in variety of skin activities.