GAD65 Polyclonal antibody
GAD65 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IF-P, IP, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | mouse brain tissue, rat brain tissue, mouse cerebellum tissue, rat brain brain tissue |
Positive IP detected in | rat brain tissue |
Positive IHC detected in | mouse brain tissue, human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | mouse brain tissue |
Positive IF/ICC detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:100-1:3000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:200-1:500 |
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 4 publications below |
产品信息
21760-1-AP targets GAD65 in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, IF/ICC, IF-P, IP, ELISA Application Description |
Cited Applications | WB |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Immunogen | GAD65 fusion protein Ag16251 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa) |
Synonyms | GAD2, GAD-65, GAD 65, EC:4.1.1.15 |
Calculated Molecular Weight | 585 aa, 65 kDa |
Observed Molecular Weight | 65 kDa |
GenBank Accession Number | BC126327 |
Gene Symbol | GAD65 |
Gene ID (NCBI) | 2572 |
RRID | AB_2878916 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q05329 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for GAD65 antibody 21760-1-AP | Download protocol |
IHC protocol for GAD65 antibody 21760-1-AP | Download protocol |
IF protocol for GAD65 antibody 21760-1-AP | Download protocol |
IP protocol for GAD65 antibody 21760-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Sci Adv RasGRP1 is a causal factor in the development of l-DOPA-induced dyskinesia in Parkinson's disease. | ||
J Neuroinflammation FKBP51 mediates resilience to inflammation-induced anxiety through regulation of glutamic acid decarboxylase 65 expression in mouse hippocampus. | ||
Neuropharmacology Inhibition of Cdk5 rejuvenates inhibitory circuits and restores experience-dependent plasticity in adult visual cortex. | ||
Neurosci Lett Repeated maternal separation prolongs the critical period of ocular dominance plasticity in mouse visual cortex. |