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GADD45A Recombinant antibody, PBS Only (Detector)

GADD45A Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241597F5

Cat no : 84376-3-PBS

Print datasheet

Synonyms

GADD45, DNA damage-inducible transcript 1 protein, DDIT-1, DDIT1, DDIT 1

验证数据展示

  • IHC staining of human stomach cancer using 84376-3-RR (same clone as 84376-3-PBS)

    IHC staining of human stomach cancer using 84376-3-RR (same clone as 84376-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 84376-3-RR (GADD45A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84376-3-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 84376-3-RR (same clone as 84376-3-PBS)

    IHC staining of human stomach cancer using 84376-3-RR (same clone as 84376-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 84376-3-RR (GADD45A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84376-3-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 84376-3-RR (same clone as 84376-3-PBS)

    IF Staining of U2OS using 84376-3-RR (same clone as 84376-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using GADD45A antibody (84376-3-RR, Clone: 241597F5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84376-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01247-3

    Cytometric bead array standard curve of MP01247-3

    Cytometric bead array standard curve of MP01247-3, GADD45A Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84376-2-PBS. Detection antibody: 84376-3-PBS. Standard: Ag4687. Range: 0.313-40 ng/mL.

  • Affinity and Kinetic Characterization of 84376-3-RR

    Affinity and Kinetic Characterization of 84376-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84376-3-RR against Human GADD45A were performed. The affinity constant is below 1 pM.

产品信息

84376-3-PBS targets GADD45A as part of a matched antibody pair:

MP01247-3: 84376-2-PBS capture and 84376-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications IHC, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human
Immunogen GADD45A fusion protein Ag4687 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name growth arrest and DNA-damage-inducible, alpha
Synonyms GADD45, DNA damage-inducible transcript 1 protein, DDIT-1, DDIT1, DDIT 1
Calculated Molecular Weight 165 aa, 18 kDa
GenBank Accession NumberBC011757
Gene Symbol GADD45A
Gene ID (NCBI) 1647
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP24522
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C