GADD45GIP1 Polyclonal antibody

GADD45GIP1 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 16260-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (1)

应用

WB, IHC, IP, ELISA and More (1)

Growth arrest and DNA damage-inducible proteins-interacting protein 1, CRIF1, CR6-interacting factor 1, CKII beta-associating protein, CKBBP2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHeLa cells, NIH/3T3 cells, mouse heart tissue, HEK-293 cells, SKOV-3 cells
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman ovary tumor tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy GADD45GIP1 Ready-To-Use IHC Kit. GADD45GIP1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16260-1-AP targets GADD45GIP1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IP, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse
文献引用反应性human, mouse, rat
免疫原 GADD45GIP1 fusion protein Ag9299 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 growth arrest and DNA-damage-inducible, gamma interacting protein 1
别名 Growth arrest and DNA damage-inducible proteins-interacting protein 1, CRIF1, CR6-interacting factor 1, CKII beta-associating protein, CKBBP2
计算分子量 222 aa, 25 kDa
观测分子量 25-28 kDa
GenBank蛋白编号BC013027
基因名称 GADD45GIP1
Gene ID (NCBI) 90480
RRIDAB_2108229
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8TAE8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GADD45GIP1 antibody 16260-1-APDownload protocol
IHC protocol for GADD45GIP1 antibody 16260-1-APDownload protocol
IP protocol for GADD45GIP1 antibody 16260-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IF

Science

T cell metabolism. The protein LEM promotes CD8⁺ T cell immunity through effects on mitochondrial respiration.

Authors - Isobel Okoye
humanIHC

Oncotarget

CR6-interacting factor 1 inhibits invasiveness by suppressing TGF-β-mediated epithelial-mesenchymal transition in hepatocellular carcinoma.

Authors - Runzhou Zhuang
ratWB

J Mol Med (Berl)

Effect of DJ-1 on the neuroprotection of astrocytes subjected to cerebral ischemia/reperfusion injury.

Authors - Li Peng
mouseWB

Burns Trauma

Cardiac proteomics reveals the potential mechanism of microtubule associated protein 4 phosphorylation-induced mitochondrial dysfunction.

Authors - Lingfei Li
mouseWB,IHC

Int J Mol Sci

Nmnat1 Deficiency Causes Mitoribosome Excess in Diabetic Nephropathy Mediated by Transcriptional Repressor HIC1

Authors - Kazuhiro Hasegawa
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