GADD45GIP1 Polyclonal antibody

GADD45GIP1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 16260-1-AP

Print datasheet

Synonyms

CKBBP2, CKII beta associating protein, CR6 interacting factor 1, CRIF1, GADD45GIP1, p53 responsive gene 6 protein, PLINP 1, Plinp1, PRG6



经过测试的应用

Positive WB detected inHeLa cells, NIH/3T3 cells, mouse heart tissue, HEK-293 cells, SKOV-3 cells
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16260-1-AP targets GADD45GIP1 in WB, IHC, IP, ELISA, IF applications and shows reactivity with human, mouse samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Immunogen GADD45GIP1 fusion protein Ag9299 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name growth arrest and DNA-damage-inducible, gamma interacting protein 1
Synonyms CKBBP2, CKII beta associating protein, CR6 interacting factor 1, CRIF1, GADD45GIP1, p53 responsive gene 6 protein, PLINP 1, Plinp1, PRG6
Calculated Molecular Weight 222 aa, 25 kDa
Observed Molecular Weight 25-28 kDa
GenBank Accession NumberBC013027
Gene Symbol GADD45GIP1
Gene ID (NCBI) 90480
RRIDAB_2108229
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TAE8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GADD45GIP1 antibody 16260-1-APDownload protocol
IHC protocol for GADD45GIP1 antibody 16260-1-APDownload protocol
IP protocol for GADD45GIP1 antibody 16260-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IF

Science

T cell metabolism. The protein LEM promotes CD8⁺ T cell immunity through effects on mitochondrial respiration.

Authors - Isobel Okoye
humanIHC

Oncotarget

CR6-interacting factor 1 inhibits invasiveness by suppressing TGF-β-mediated epithelial-mesenchymal transition in hepatocellular carcinoma.

Authors - Runzhou Zhuang
ratWB

J Mol Med (Berl)

Effect of DJ-1 on the neuroprotection of astrocytes subjected to cerebral ischemia/reperfusion injury.

Authors - Li Peng
mouseWB

Burns Trauma

Cardiac proteomics reveals the potential mechanism of microtubule associated protein 4 phosphorylation-induced mitochondrial dysfunction.

Authors - Lingfei Li
mouseWB,IHC

Int J Mol Sci

Nmnat1 Deficiency Causes Mitoribosome Excess in Diabetic Nephropathy Mediated by Transcriptional Repressor HIC1

Authors - Kazuhiro Hasegawa