验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, NIH/3T3 cells, mouse heart tissue, HEK-293 cells, SKOV-3 cells |
Positive IP detected in | mouse heart tissue |
Positive IHC detected in | human ovary tumor tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
16260-1-AP targets GADD45GIP1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.
经测试应用 | WB, IHC, IP, ELISA Application Description |
文献引用应用 | WB, IHC, IF |
经测试反应性 | human, mouse |
文献引用反应性 | human, mouse, rat |
免疫原 | GADD45GIP1 fusion protein Ag9299 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | growth arrest and DNA-damage-inducible, gamma interacting protein 1 |
别名 | Growth arrest and DNA damage-inducible proteins-interacting protein 1, CRIF1, CR6-interacting factor 1, CKII beta-associating protein, CKBBP2 |
计算分子量 | 222 aa, 25 kDa |
观测分子量 | 25-28 kDa |
GenBank蛋白编号 | BC013027 |
基因名称 | GADD45GIP1 |
Gene ID (NCBI) | 90480 |
RRID | AB_2108229 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q8TAE8 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for GADD45GIP1 antibody 16260-1-AP | Download protocol |
IHC protocol for GADD45GIP1 antibody 16260-1-AP | Download protocol |
IP protocol for GADD45GIP1 antibody 16260-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Science T cell metabolism. The protein LEM promotes CD8⁺ T cell immunity through effects on mitochondrial respiration. | ||
Oncotarget CR6-interacting factor 1 inhibits invasiveness by suppressing TGF-β-mediated epithelial-mesenchymal transition in hepatocellular carcinoma. | ||
J Mol Med (Berl) Effect of DJ-1 on the neuroprotection of astrocytes subjected to cerebral ischemia/reperfusion injury. | ||
Burns Trauma Cardiac proteomics reveals the potential mechanism of microtubule associated protein 4 phosphorylation-induced mitochondrial dysfunction. | ||
Int J Mol Sci Nmnat1 Deficiency Causes Mitoribosome Excess in Diabetic Nephropathy Mediated by Transcriptional Repressor HIC1 |