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验证数据展示

  • WB analysis using 83823-5-RR

    WB analysis using 83823-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83823-5-RR (GADD45GIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovarian cancer using 83823-5-RR

    IHC staining of human ovarian cancer using 83823-5-RR

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83823-5-RR (GADD45GIP1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovarian cancer using 83823-5-RR

    IHC staining of human ovarian cancer using 83823-5-RR

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 83823-5-RR (GADD45GIP1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

GADD45GIP1 Recombinant antibody

GADD45GIP1 Recombinant Antibody for WB, IHC, ELISA
Cat No. 83823-5-RR

Print datasheet

CloneNo. 240947D1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

CRIF1, CR6-interacting factor 1, CKII beta-associating protein, CKBBP2, 39S ribosomal protein L59, mitochondrial

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inSKOV-3 cells, Jurkat cells, U-251 cells
Positive IHC detected inhuman ovarian cancer
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83823-5-RR targets GADD45GIP1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen GADD45GIP1 fusion protein Ag9299 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name growth arrest and DNA-damage-inducible, gamma interacting protein 1
Synonyms CRIF1, CR6-interacting factor 1, CKII beta-associating protein, CKBBP2, 39S ribosomal protein L59, mitochondrial
Calculated Molecular Weight 222 aa, 25 kDa
Observed Molecular Weight 25-28 kDa
GenBank Accession NumberBC013027
Gene Symbol GADD45GIP1
Gene ID (NCBI) 90480
RRIDAB_3671407
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ8TAE8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Growth arrest and DNA-damage-inducible proteins-interacting protein 1(GADD45GIP1), also known as CRIF1, is newly identified de novo components in large subunit of mitoribosome. It is essential for the translation of mitochondrial oxidative phosphorylation (OXPHOS) polypeptides in mammalian mitochondria. The essential role of CRIF1 in mitochondrial synthesis and membrane integration of OXPHOS polypeptides was shown in brain-specific CRIF1-deficient mice, which exhibited profound OXPHOS failure and marked neurodegeneration. (PMID 22819524) In addition, GADD45GIP1 enhances the function of GADD45 in maintaining genome stability, DNA repair and cell cycle regulation by interacting with the GADD45 protein family. It's reported that GADD45GIP1 may be induced by p53, a process that is closely related to the cell's response to DNA damage.(PMID:28599472)

实验方案

Product Specific Protocols
WB protocol for GADD45GIP1 antibody 83823-5-RRDownload protocol
IHC protocol for GADD45GIP1 antibody 83823-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols