GADD45GIP1 Recombinant antibody
GADD45GIP1 Uni-rAbTM Recombinant Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
240947D1
验证数据展示
经过测试的应用
| Positive WB detected in | SKOV-3 cells, Jurkat cells, U-251 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
The immunogen of 83823-5-RR is GADD45GIP1 Fusion Protein expressed in E. coli.
| Tested Applications | WB, ELISA Application Description |
| Tested Reactivity | human |
| Immunogen | GADD45GIP1 fusion protein Ag9299 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | growth arrest and DNA-damage-inducible, gamma interacting protein 1 |
| Synonyms | CRIF1, CR6-interacting factor 1, CKII beta-associating protein, CKBBP2, 39S ribosomal protein L59, mitochondrial |
| Calculated Molecular Weight | 222 aa, 25 kDa |
| Observed Molecular Weight | 25-28 kDa |
| GenBank Accession Number | BC013027 |
| Gene Symbol | GADD45GIP1 |
| Gene ID (NCBI) | 90480 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | Q8TAE8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Growth arrest and DNA-damage-inducible proteins-interacting protein 1(GADD45GIP1), also known as CRIF1, is newly identified de novo components in large subunit of mitoribosome. It is essential for the translation of mitochondrial oxidative phosphorylation (OXPHOS) polypeptides in mammalian mitochondria. The essential role of CRIF1 in mitochondrial synthesis and membrane integration of OXPHOS polypeptides was shown in brain-specific CRIF1-deficient mice, which exhibited profound OXPHOS failure and marked neurodegeneration. (PMID 22819524) In addition, GADD45GIP1 enhances the function of GADD45 in maintaining genome stability, DNA repair and cell cycle regulation by interacting with the GADD45 protein family. It's reported that GADD45GIP1 may be induced by p53, a process that is closely related to the cell's response to DNA damage.(PMID:28599472)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GADD45GIP1 antibody 83823-5-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |