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GAK Polyclonal antibody

GAK Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 12147-1-AP

Print datasheet

Synonyms

EC:2.7.11.1, DNAJC26

验证数据展示

  • WB analysis of human brain using 12147-1-AP

    WB analysis of human brain using 12147-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 12147-1-AP (GAK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 12147-1-AP

    IHC staining of mouse brain using 12147-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12147-1-AP (GAK antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GAK antibody included
  • IF Staining of HepG2 using 12147-1-AP

    IF Staining of HepG2 using 12147-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 12147-1-AP (GAK antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inhuman brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy GAK Ready-To-Use IHC Kit. GAK primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

12147-1-AP targets GAK in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen GAK fusion protein Ag2795 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cyclin G associated kinase
Synonyms EC:2.7.11.1, DNAJC26
Calculated Molecular Weight 134 kDa, 143 kDa
Observed Molecular Weight 150 kDa
GenBank Accession NumberBC008668
Gene Symbol GAK
Gene ID (NCBI) 2580
RRIDAB_2108368
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO14976
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GAK(cyclin-G-associated kinase) belongs to the protein kinase superfamily. GAK plays a more positive role as a modulator of signal transduction, by relaying the phosphorylation signal from an unidentified tyrosine kinase to unidentified GAK phosphorylation targets(PMID:9013862). It may be acting as an auxilin homolog that is involved in the uncoating of clathrin- coated vesicles by hsc70 in non-neuronal cells.

实验方案

Product Specific Protocols
WB protocol for GAK antibody 12147-1-APDownload protocol
IHC protocol for GAK antibody 12147-1-APDownload protocol
IF protocol for GAK antibody 12147-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Biol Chem

Development of the non-receptor tyrosine kinase FER-targeting PROTACs as a potential strategy for antagonizing ovarian cancer cell motility and invasiveness

Authors - Yanchun Zhang
View Article