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GALE Monoclonal antibody

GALE Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat, Pig, Rabbit

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

3F2C2

Cat no : 67772-1-Ig

Print datasheet

Synonyms

FLJ95174, FLJ97302, Galactowaldenase, GALE, SDR1E1, UDP galactose 4 epimerase, UDP glucose 4 epimerase

验证数据展示

  • WB analysis using 67772-1-Ig

    WB analysis using 67772-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67772-1-Ig (GALE antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inU2OS cells, A375 cells, HEK-293T cells, HeLa cells, K-562 cells, 4T1 cells, rat liver tissue, pig liver tissue, rabbit liver tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67772-1-Ig targets GALE in WB, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig, Rabbit samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, Mouse, Rat, Pig, Rabbit
Immunogen GALE fusion protein Ag6571 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name UDP-galactose-4-epimerase
Synonyms FLJ95174, FLJ97302, Galactowaldenase, GALE, SDR1E1, UDP galactose 4 epimerase, UDP glucose 4 epimerase
Calculated Molecular Weight 38 kDa
Observed Molecular Weight 38 kDa
GenBank Accession NumberBC050685
Gene Symbol GALE
Gene ID (NCBI) 2582
RRIDAB_2918537
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ14376
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GALE(Galactowaldenase) is also named as UDP-glucose 4-epimerase, UDP-galactose 4-epimerase, and belongs to the sugar epimerase family. UDP-galactose 4-epimerase catalyzes the interconversion of UDP-glucose and UDP-galactose during normal galactose metabolism. In humans, deficiencies in this enzyme lead to the complex disorder referred to as epimerase-deficiency galactosemia(PMID:10801319).It can exsit as a homodimer(PMID:8931134).

实验方案

Product Specific Protocols
WB protocol for GALE antibody 67772-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols