GALNS Polyclonal antibody
GALNS Polyclonal Antibody for IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
IHC, WB, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | MCF-7 cells, human liver tissue, HepG2 cells, HeLa cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IHC | See 1 publications below |
| IF | See 1 publications below |
产品信息
14933-1-AP targets GALNS in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | IHC, WB, ELISA Application Description |
| Cited Applications | IF, IHC |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Immunogen | GALNS fusion protein Ag6746 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | galactosamine (N-acetyl)-6-sulfate sulfatase |
| Synonyms | Chondroitinase, Chondroitinsulfatase, Galactose 6 sulfate sulfatase, GALNAC6S, GalNAc6S sulfatase, GALNS, GAS, MPS4A |
| Calculated Molecular Weight | 58 kDa |
| Observed Molecular Weight | 50-55 kDa |
| GenBank Accession Number | BC050684 |
| Gene Symbol | GALNS |
| Gene ID (NCBI) | 2588 |
| RRID | AB_2108720 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P34059 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GALNS(N-acetylgalactosamine-6-sulfatase) is also named as chondroitinase and belongs to the sulfatase family. It is one of sulfatases required to degrade glycosaminoglycans (GAGs), keratan sulfate (KS) and chondroitin-6-sulfate (C6S) and the enzyme is a dimer derived from two 60 kDa polypeptides, each of which is processed to 40 kDa and 15 kDa polypeptide subunits linked by disulfide bonds(PMID:15241807). The deduced 522-residue protein is composed of a 26-amino acid N-terminal signal peptide and a mature polypeptide of 496 amino acid residues, including 2 potential asparagine-linked glycosylation sites(PMID:1755850). Defects in GALNS are the cause of mucopolysaccharidosis type 4A (MPS4A), also known as Morquio A syndrome.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GALNS antibody 14933-1-AP | Download protocol |
| IHC protocol for GALNS antibody 14933-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Discov Oncol Identification of galactosamine-(N-acetyl)-6-sulfatase (GALNS) as a novel therapeutic target in progression of nasopharyngeal carcinoma |
