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GALNT1 Polyclonal antibody

GALNT1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 21139-1-AP

Print datasheet

Synonyms

GALNAC T1, GALNT1, pp GaNTase 1

验证数据展示

  • WB analysis using 21139-1-AP

    WB analysis using 21139-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21139-1-AP (GALNT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inBGC-823 cells, HepG2 cells, HuH-7 cells, MKN-45 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21139-1-AP targets GALNT1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen GALNT1 fusion protein Ag15591 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 1 (GalNAc-T1)
Synonyms GALNAC T1, GALNT1, pp GaNTase 1
Calculated Molecular Weight 559 aa, 64 kDa
Observed Molecular Weight64 kDa
GenBank Accession NumberBC038440
Gene Symbol GALNT1
Gene ID (NCBI) 2589
RRIDAB_3085639
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ10472
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

N-acetylgalactosaminyltransferase 1 (GALNT1) is a member of a large family of Golgi resident polypeptide GALNT that initiate mucin-type O-glycosylation by transfer of α-GalNAc from UDP-GalNAc to serine or threonine residues of proteins. GALNT1 is frequently up-regulated in HCC and gastric cancer, and higher GALNT1 expression levels are associated with poorer overall survival (PMID: 25730904, 36439876). The antibody against GALNT1 recognized a specific band at 64 kDa.

实验方案

Product Specific Protocols
WB protocol for GALNT1 antibody 21139-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols