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GALNT10 Polyclonal antibody

GALNT10 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20822-1-AP

Print datasheet

Synonyms

GalNAc T10, GalNAcT10, GALNT10, pp GalNAc T10, pp GaNTase 10

验证数据展示

  • WB analysis using 20822-1-AP

    WB analysis using 20822-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20822-1-AP (GALNT10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 20822-1-AP

    IHC staining of human liver cancer using 20822-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20822-1-AP (GALNT10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inSK-N-SH cells, HepG2 cells, mouse ovary tissue
Positive IHC detected inhuman liver cancer tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20822-1-AP targets GALNT10 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen GALNT10 fusion protein Ag14751 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 10 (GalNAc-T10)
Synonyms GalNAc T10, GalNAcT10, GALNT10, pp GalNAc T10, pp GaNTase 10
Calculated Molecular Weight 603 aa, 69 kDa
Observed Molecular Weight62 kDa
GenBank Accession NumberBC007224
Gene Symbol GALNT10
Gene ID (NCBI) 55568
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86SR1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GALNT10 is a member of the N-acetylgalactosyltransferase family and an enzyme that catalyzes the first step in O-glycan synthesis. O-glycosylation is closely related to cell recognition, tumor cell growth, metastasis and adhesion. GALNT10 can change the cell connection and communication environment between cells, thus achieving specific biological effects. GALNT10 is highly expressed in many tumors and the tumor migration and invasion ability can be suppressed by inhibiting the expression of GALNT10 (PMID: 33275228). GALNT10 is a prognostic biomarker of high-grade ovarian serous cancer (HGSC) patients (PMID: 31853576). GALNT10 has 4 isoforms with the molecular mass of 32, 41, 62 and 69 kDa.

实验方案

Product Specific Protocols
WB protocol for GALNT10 antibody 20822-1-APDownload protocol
IHC protocol for GALNT10 antibody 20822-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols