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验证数据展示

  • WB analysis using 20822-1-AP

    WB analysis using 20822-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20822-1-AP (GALNT10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 20822-1-AP

    IHC staining of human liver cancer using 20822-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20822-1-AP (GALNT10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

GALNT10 Polyclonal antibody

GALNT10 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 20822-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

GalNAc T10, GalNAcT10, GALNT10, pp GalNAc T10, pp GaNTase 10

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inSK-N-SH cells, HepG2 cells, mouse ovary tissue
Positive IHC detected inhuman liver cancer tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20822-1-AP targets GALNT10 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 GALNT10 fusion protein Ag14751 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 10 (GalNAc-T10)
别名 GalNAc T10, GalNAcT10, GALNT10, pp GalNAc T10, pp GaNTase 10
计算分子量 603 aa, 69 kDa
观测分子量62 kDa
GenBank蛋白编号BC007224
基因名称 GALNT10
Gene ID (NCBI) 55568
RRIDAB_3085622
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ86SR1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GALNT10 is a member of the N-acetylgalactosyltransferase family and an enzyme that catalyzes the first step in O-glycan synthesis. O-glycosylation is closely related to cell recognition, tumor cell growth, metastasis and adhesion. GALNT10 can change the cell connection and communication environment between cells, thus achieving specific biological effects. GALNT10 is highly expressed in many tumors and the tumor migration and invasion ability can be suppressed by inhibiting the expression of GALNT10 (PMID: 33275228). GALNT10 is a prognostic biomarker of high-grade ovarian serous cancer (HGSC) patients (PMID: 31853576). GALNT10 has 4 isoforms with the molecular mass of 32, 41, 62 and 69 kDa.

实验方案

Product Specific Protocols
WB protocol for GALNT10 antibody 20822-1-APDownload protocol
IHC protocol for GALNT10 antibody 20822-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols