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验证数据展示

  • IHC staining of mouse pancreas using 17441-1-AP

    IHC staining of mouse pancreas using 17441-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 17441-1-AP (GALNT2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GALNT2 antibody included
  • WB analysis using 17441-1-AP

    WB analysis using 17441-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17441-1-AP (GALNT2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 17441-1-AP

    IHC staining of human colon using 17441-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 17441-1-AP (GALNT2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GALNT2 antibody included
  • IF Staining of HeLa using 17441-1-AP

    IF Staining of HeLa using 17441-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GALNT2 antibody (17441-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

  • IF Staining of HeLa using 17441-1-AP

    IF Staining of HeLa using 17441-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GALNT2 antibody (17441-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

GALNT2 Polyclonal antibody

GALNT2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 17441-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

pp GaNTase 2, Polypeptide N-acetylgalactosaminyltransferase 2 soluble form, Polypeptide N-acetylgalactosaminyltransferase 2, GalNAc-T2, GalNAc T2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 594
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, HeLa cells, MKN-45 cells
Positive IHC detected inmouse pancreas tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy GALNT2 Ready-To-Use IHC Kit. GALNT2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

17441-1-AP targets GALNT2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用IHC
经测试反应性 human, mouse
文献引用反应性human
免疫原 GALNT2 fusion protein Ag11523 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 2 (GalNAc-T2)
别名 pp GaNTase 2, Polypeptide N-acetylgalactosaminyltransferase 2 soluble form, Polypeptide N-acetylgalactosaminyltransferase 2, GalNAc-T2, GalNAc T2
计算分子量 571 aa, 65 kDa
观测分子量64 kDa
GenBank蛋白编号BC041120
基因名称 GALNT2
Gene ID (NCBI) 2590
RRIDAB_3085523
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ10471
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GALNT2, a GalNAc-transferase involved in O-linked glycosylation, modulates the risk of atherogenic dyslipidemia, a specific hallmark of insulin resistance and acts as a positive modulator of insulin signaling in human liver cells by down-regulating ENPP1. GALNT2 gene encodes GalNAc-T2 as a regulator of high-density lipoprotein cholesterol (HDL-C) metabolism (PMID: 31040393, 27508872). Western blot analysis detected GALNT2 at an apparent molecular mass of 64 kDa.

实验方案

Product Specific Protocols
WB protocol for GALNT2 antibody 17441-1-APDownload protocol
IHC protocol for GALNT2 antibody 17441-1-APDownload protocol
IF protocol for GALNT2 antibody 17441-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Cell Biol Toxicol

GALNT2, an O-glycosylating enzyme, is a critical regulator of radioresistance of non-small cell lung cancer: evidence from an integrated multi-omics analysis

Authors - Xiaoxia Dong
View Article