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验证数据展示

  • WB analysis using 29909-1-AP

    WB analysis using 29909-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29909-1-AP (GALR2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 29909-1-AP

    IHC staining of human pancreas cancer using 29909-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 29909-1-AP (GALR2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GALR2 antibody included
  • IHC staining of human pancreas cancer using 29909-1-AP

    IHC staining of human pancreas cancer using 29909-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 29909-1-AP (GALR2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GALR2 antibody included
  • IF Staining of HepG2 using 29909-1-AP

    IF Staining of HepG2 using 29909-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using GALR2 antibody (29909-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

GALR2 Polyclonal antibody

GALR2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 29909-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

GALR 2, GALNR2, Galanin receptor type 2, galanin receptor 2, GAL2-R

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHepG2 cells, SMMC-7721 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy GALR2 Ready-To-Use IHC Kit. GALR2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29909-1-AP targets GALR2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 GALR2 fusion protein Ag31762 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 galanin receptor 2
别名 GALR 2, GALNR2, Galanin receptor type 2, galanin receptor 2, GAL2-R
计算分子量42KD
观测分子量 42-50 kDa
GenBank蛋白编号NM_003857
基因名称 GALR2
Gene ID (NCBI) 8811
RRIDAB_2935488
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO43603
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Galanin receptor type 2 (GALR2) is a receptor for galanin, GALP, and the hormone SPX1 (PMID:24517231). It is an important neuromodulator present in the brain, gastrointestinal system, and hypothalamo-pituitary axis. Activation of the GALR2 may protect the hippocampus from neuronal damage (PMID: 17263796). It is a 30-amino acid non-C-terminally amidated peptide that potently stimulates growth hormone secretion, inhibits cardiac vagal slowing of heart rate, abolishes sinus arrhythmia, and inhibits postprandial gastrointestinal motility. The activity of this receptor is mediated by G proteins that activate the phospholipase C/protein kinase C pathway (via G(q)) and that inhibits adenylyl cyclase. GALR2 has a calculated molecular weight of 42 kDa.

实验方案

Product Specific Protocols
WB protocol for GALR2 antibody 29909-1-APDownload protocol
IHC protocol for GALR2 antibody 29909-1-APDownload protocol
IF protocol for GALR2 antibody 29909-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols