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GALT Polyclonal antibody

GALT Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17035-1-AP

Print datasheet

Synonyms

Gal 1 P uridylyltransferase, GALT

验证数据展示

  • WB analysis of human liver using 17035-1-AP

    WB analysis of human liver using 17035-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 17035-1-AP (GALT antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 17035-1-AP

    IHC staining of human liver using 17035-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 17035-1-AP (GALT antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human liver using 17035-1-AP

    IHC staining of human liver using 17035-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 17035-1-AP (GALT antibody) at dilution of 1:100 (under 40x lens).

经过测试的应用

Positive WB detected inhuman liver tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

17035-1-AP targets GALT in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen GALT fusion protein Ag10524 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name galactose-1-phosphate uridylyltransferase
Synonyms Gal 1 P uridylyltransferase, GALT
Calculated Molecular Weight 379 aa, 43 kDa
Observed Molecular Weight 45-48 kDa
GenBank Accession NumberBC015045
Gene Symbol GALT
Gene ID (NCBI) 2592
RRIDAB_2109296
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP07902
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GALT (galactose-1-phosphate uridylyltransferase) catalyses the second step in the Leloir pathway for the conversion of galactose-l-phosphate (Gal-I-P) into uridine diphosphogalactose (UDP-gal). Defects in GALT cause galactosemia.

实验方案

Product Specific Protocols
WB protocol for GALT antibody 17035-1-APDownload protocol
IHC protocol for GALT antibody 17035-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Inherit Metab Dis

Galactose 1-phosphate accumulates to high levels in galactose-treated cells due to low GALT activity and absence of product inhibition of GALK.

Authors - Sher Li Oh
View Article
humanWB

PLoS One

Angelica Sinensis Polysaccharides Stimulated UDP-Sugar Synthase Genes through Promoting Gene Expression of IGF-1 and IGF1R in Chondrocytes: Promoting Anti-Osteoarthritic Activity.

Authors - Yinxian Wen
View Article