验证数据展示
经过测试的应用
| Positive WB detected in | fetal human brain tissue, mouse liver tissue, pig liver tissue, rat liver tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 2 publications below |
| WB | See 4 publications below |
产品信息
66322-1-Ig targets GAMT in WB, ELISA applications and shows reactivity with human, rat, mouse, pig samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, rat, mouse, pig |
| 文献引用反应性 | human, mouse |
| 免疫原 | GAMT fusion protein Ag16778 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | guanidinoacetate N-methyltransferase |
| 别名 | GAMT, PIG2, TP53I2 |
| 计算分子量 | 26 kDa |
| 观测分子量 | 26 kDa |
| GenBank蛋白编号 | BC016760 |
| 基因名称 | GAMT |
| Gene ID (NCBI) | 2593 |
| RRID | AB_2881702 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q14353 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GAMT converts the latter to creatine with S-adenosylmethionine as the methyl donor. GAMT was induced in human and mouse cells in response to genotoxic and metabolic stress. GAMT was required for p53-dependent upregulation of creatine biosynthesis, fatty acid oxidation, and apoptosis in response to glucose deprivation.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GAMT antibody 66322-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Food Funct The protective effect of polyunsaturated fatty acid intake during pregnancy against embryotoxicity of sodium valproate in mice | ||
Sci Rep Intratesticular creatine maintains spermatogenesis by defining tight junctions
| ||
Sci Rep Identification of disulfidptosis-related subtypes in gastric cancer and GAMT is a key gene during disulfidptosis
|
