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  • Featured Product
  • KD/KO Validated

GANAB Polyclonal antibody

GANAB Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29183-1-AP

Print datasheet

Synonyms

glucosidase, alpha; neutral AB, Glucosidase II subunit alpha, G2AN, EC:3.2.1.207, Alpha-glucosidase 2

验证数据展示

  • WB analysis using 29183-1-AP

    WB analysis using 29183-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29183-1-AP (GANAB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 29183-1-AP

    WB analysis of HeLa using 29183-1-AP

    WB result of GANAB antibody (29183-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GANAB transfected HeLa cells.

  • IHC staining of mouse liver using 29183-1-AP

    IHC staining of mouse liver using 29183-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 29183-1-AP (GANAB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GANAB antibody included

经过测试的应用

Positive WB detected inmouse kidney tissue, HeLa cells, mouse liver, rat liver
Positive IHC detected inmouse liver tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy GANAB Ready-To-Use IHC Kit. GANAB primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29183-1-AP targets GANAB in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen GANAB fusion protein Ag30417 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name glucosidase, alpha; neutral AB
Synonyms glucosidase, alpha; neutral AB, Glucosidase II subunit alpha, G2AN, EC:3.2.1.207, Alpha-glucosidase 2
Calculated Molecular Weight944aa,107 kDa; 488aa,55 kDa
Observed Molecular Weight110-120 kDa
GenBank Accession NumberBC017435
Gene Symbol GANAB
Gene ID (NCBI) 23193
RRIDAB_2918244
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14697
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GANAB antibody 29183-1-APDownload protocol
IHC protocol for GANAB antibody 29183-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Glycobiology

MeCP2 regulated glycogenes contribute to proliferation and apoptosis of gastric cancer cells.

Authors - Yannan Qin
  • KD Validated
View Article