验证数据展示
经过测试的应用
| Positive WB detected in | mouse kidney tissue, HeLa cells, mouse liver, rat liver |
| Positive IHC detected in | mouse liver tissue, human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy GANAB Ready-To-Use IHC Kit. GANAB primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
产品信息
29183-1-AP targets GANAB in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 | GANAB fusion protein Ag30417 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | glucosidase, alpha; neutral AB |
| 别名 | glucosidase, alpha; neutral AB, Glucosidase II subunit alpha, G2AN, EC:3.2.1.207, Alpha-glucosidase 2 |
| 计算分子量 | 944aa,107 kDa; 488aa,55 kDa |
| 观测分子量 | 110-120 kDa |
| GenBank蛋白编号 | BC017435 |
| 基因名称 | GANAB |
| Gene ID (NCBI) | 23193 |
| RRID | AB_2918244 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q14697 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GANAB antibody 29183-1-AP | Download protocol |
| IHC protocol for GANAB antibody 29183-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
