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  • Featured Product
  • KD/KO Validated

GANP Polyclonal antibody

GANP Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 11054-1-AP

Print datasheet

Synonyms

MCM3AP, MAP80, KIAA0572, Germinal-center associated nuclear protein, EC:2.3.1.48

验证数据展示

  • WB analysis of mouse brain using 11054-1-AP

    WB analysis of mouse brain using 11054-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 11054-1-AP (GANP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 11054-1-AP

    IHC staining of human colon cancer using 11054-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 11054-1-AP (GANP antibody) at dilution of 1:10 (under 10x lens).

    Ready-to-use IHC kits available with GANP antibody included
  • IF Staining of HepG2 using 11054-1-AP

    IF Staining of HepG2 using 11054-1-AP

    Immunofluorescent analysis of HepG2 cells, using MCM3AP antibody 11054-1-AP at 1:100 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • FC experiment of HepG2 using 11054-1-AP

    FC experiment of HepG2 using 11054-1-AP

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug GANP Polyclonal antibody (11054-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IP experiment of mouse brain using 11054-1-AP

    IP experiment of mouse brain using 11054-1-AP

    IP result of anti-GANP (IP:11054-1-AP, 4ug; Detection:11054-1-AP 1:500) with mouse brain tissue lysate 4000ug.

经过测试的应用

Positive WB detected inmouse brain tissue, NIH/3T3 cells
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy GANP Ready-To-Use IHC Kit. GANP primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:10-1:100
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11054-1-AP targets GANP in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, monkey
Immunogen GANP fusion protein Ag1486 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name minichromosome maintenance complex component 3 associated protein
Synonyms MCM3AP, MAP80, KIAA0572, Germinal-center associated nuclear protein, EC:2.3.1.48
Calculated Molecular Weight 218 kDa
Observed Molecular Weight 75-80 kDa
GenBank Accession NumberBC013285
Gene Symbol GANP
Gene ID (NCBI) 8888
RRIDAB_2266280
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO60318
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The minichromosome maintenance protein 3 (MCM3) is one of the MCM proteins essential for the initiation of DNA replication. MCM3AP is MCM3 associated protein, and it has phosphorylation-dependent DNA-primase activity, which was up-regulated in antigen immunization induced germinal center. The mutagenesis of a nuclear localization signal of MCM3 affects the binding of this protein with MCM3, suggesting that MCM3AP may also facilitate MCM3 nuclear localization. Besides, MCM3AP is an acetyltransferase cotaining putative acetyl CoA binding motifs conserved within the GCN5-related N-acetyltransferase superfamily.

实验方案

Product Specific Protocols
WB protocol for GANP antibody 11054-1-APDownload protocol
IHC protocol for GANP antibody 11054-1-APDownload protocol
IF protocol for GANP antibody 11054-1-APDownload protocol
IP protocol for GANP antibody 11054-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Commun

GANP regulates recruitment of AID to immunoglobulin variable regions by modulating transcription and nucleosome occupancy.

Authors - Singh Shailendra Kumar SK
  • KD Validated
View Article
humanWB

J Immunol

GANP Interacts with APOBEC3G and Facilitates Its Encapsidation into the Virions to Reduce HIV-1 Infectivity.

Authors - Maeda Kazuhiko K
  • KD Validated
View Article
humanWB

Front Mol Neurosci

The Effect of MCM3AP-AS1/miR-211/KLF5/AGGF1 Axis Regulating Glioblastoma Angiogenesis.

Authors - Chunqing Yang
  • KD Validated
View Article
human,monkeyWB,IF

J Biol Chem

GANP-mediated recruitment of activation-induced cytidine deaminase to cell nuclei and to immunoglobulin variable region DNA.

Authors - Maeda Kazuhiko K
View Article