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验证数据展示

  • WB analysis using 84343-3-RR

    WB analysis using 84343-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84343-3-RR (GAPVD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84343-3-RR

    Affinity and Kinetic Characterization of 84343-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84343-3-RR against Human GAPVD1 were performed. The affinity constant is 1.43 nM.

GAPVD1 Recombinant antibody

GAPVD1 Recombinant Antibody for WB, ELISA
Cat No. 84343-3-RR

产品说明书

CloneNo. 241609F12

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

Rab5-activating protein 6, KIAA1521, GTPase-activating protein and VPS9 domain-containing protein 1, GAPex-5, GAPEX5

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse heart tissue, rat heart tissue
Planning an IHC experiment? We recommend our IHCeasy GAPVD1 Ready-To-Use IHC Kit. GAPVD1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84343-3-RR targets GAPVD1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 GAPVD1 fusion protein Ag10492 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 GTPase activating protein and VPS9 domains 1
别名 Rab5-activating protein 6, KIAA1521, GTPase-activating protein and VPS9 domain-containing protein 1, GAPex-5, GAPEX5
计算分子量 1478 aa, 165 kDa
观测分子量200-210 kDa
GenBank蛋白编号BC021119
基因名称 GAPVD1
Gene ID (NCBI) 26130
RRIDAB_3671883
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ14C86
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GTPase activating protein and VPS9 domains 1(GAPVD1) is a multifunctional protein involved in cellular processes such as endocytosis, insulin receptor internalization, and GLUT4 trafficking. Its function is critical in maintaining cellular homeostasis and it interacts with various proteins, including those involved in endocytosis and circadian rhythm regulation.

实验方案

Product Specific Protocols
WB protocol for GAPVD1 antibody 84343-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols