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- KD/KO Validated
GAR1 Polyclonal antibody
GAR1 Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IP, IHC, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | A375 cells, human skin tissue |
| Positive IP detected in | A375 cells |
| Positive IHC detected in | human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11711-1-AP targets GAR1 in WB, IHC, IP, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, IP, IHC, ELISA Application Description |
| Cited Applications | WB, IHC, IP, CoIP |
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Immunogen | GAR1 fusion protein Ag2282 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | GAR1 ribonucleoprotein homolog (yeast) |
| Synonyms | GAR1, NOLA1, snoRNP protein GAR1 |
| Calculated Molecular Weight | 217 aa, 22 kDa |
| Observed Molecular Weight | 28 kDa |
| GenBank Accession Number | BC003413 |
| Gene Symbol | GAR1 |
| Gene ID (NCBI) | 54433 |
| RRID | AB_2081845 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NY12 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GAR1, other named NOLA1, is a subunit of H/ACA and telomerase. Thought is not required for H/ACA protein assembly, GAR1 is necessary for ribosome biogenesis and telomere. H/ACA involves in class specify the sites of uridine-to-pseudouridine. It contains a core domain that flanked by glycine- and arginine-rich(GAR) domains. GAR1 also required for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transciptase(TERT) holoenzyme.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GAR1 antibody 11711-1-AP | Download protocol |
| IHC protocol for GAR1 antibody 11711-1-AP | Download protocol |
| IP protocol for GAR1 antibody 11711-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cell The human base excision repair enzyme SMUG1 directly interacts with DKC1 and contributes to RNA quality control. | ||
Genome Biol Human dyskerin binds to cytoplasmic H/ACA-box-containing transcripts affecting nuclear hormone receptor dependence | ||
Front Cell Dev Biol PARP1 Regulates the Biogenesis and Activity of Telomerase Complex Through Modification of H/ACA-Proteins. | ||
Mol Cell Biol SUMOylation- and GAR1-Dependent Regulation of Dyskerin Nuclear and Subnuclear Localization. | ||
Pathology Expressional analysis of NOLA1, NOLA2, NOLA3 and DKC1, the core proteins in H/ACA riboproteins, in gastric and colorectal cancers. | ||
Histochem Cell Biol Differential requirements for H/ACA ribonucleoprotein components in cell proliferation and response to DNA damage. |
