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验证数据展示

  • WB analysis using 83577-4-RR

    WB analysis using 83577-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83577-4-RR (GAR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • FC experiment of K562 using 83577-4-RR

    FC experiment of K562 using 83577-4-RR

    1x10^6 K562 were intracellularly stained with 0.2 ug GAR1 Recombinant antibody (83577-4-RR, Clone:240537A2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.2 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83577-4-RR

    Affinity and Kinetic Characterization of 83577-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83577-4-RR against Human GAR1 were performed. The affinity constant is 1.18 nM.

GAR1 Recombinant antibody

GAR1 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83577-4-RR

产品说明书

CloneNo. 240537A2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, FC (Intra), ELISA

别名

snoRNP protein GAR1, NOLA1, 240537A2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA375 cells, HeLa cells, mouse skin tissue
Positive FC (Intra) detected inK562

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83577-4-RR targets GAR1 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原 GAR1 fusion protein Ag2282 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 GAR1 ribonucleoprotein homolog (yeast)
别名 snoRNP protein GAR1, NOLA1, 240537A2
计算分子量 217 aa, 22 kDa
观测分子量 25-28 kDa
GenBank蛋白编号BC003413
基因名称 GAR1
Gene ID (NCBI) 54433
RRIDAB_3671189
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ9NY12
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GAR1, other named NOLA1, is a subunit of H/ACA and telomerase. Thought is not required for H/ACA protein assembly, GAR1 is necessary for ribosome biogenesis and telomere. H/ACA involves in class specify the sites of uridine-to-pseudouridine. It contains a core domain that flanked by glycine- and arginine-rich(GAR) domains. GAR1 also required for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transciptase(TERT) holoenzyme.

实验方案

Product Specific Protocols
WB protocol for GAR1 antibody 83577-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols