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GAR1 Recombinant antibody

GAR1 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240537A2

Cat no : 83577-4-RR

Print datasheet

Synonyms

snoRNP protein GAR1, NOLA1, 240537A2

验证数据展示

  • WB analysis using 83577-4-RR

    WB analysis using 83577-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83577-4-RR (GAR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • FC experiment of K562 using 83577-4-RR

    FC experiment of K562 using 83577-4-RR

    1x10^6 K562 were intracellularly stained with 0.2 ug GAR1 Recombinant antibody (83577-4-RR, Clone:240537A2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.2 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 83577-4-RR

    Affinity and Kinetic Characterization of 83577-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83577-4-RR against Human GAR1 were performed. The affinity constant is 1.18 nM.

经过测试的应用

Positive WB detected inA375 cells, HeLa cells, mouse skin tissue
Positive FC (Intra) detected inK562

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83577-4-RR targets GAR1 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse
Immunogen GAR1 fusion protein Ag2282 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name GAR1 ribonucleoprotein homolog (yeast)
Synonyms snoRNP protein GAR1, NOLA1, 240537A2
Calculated Molecular Weight 217 aa, 22 kDa
Observed Molecular Weight 25-28 kDa
GenBank Accession NumberBC003413
Gene Symbol GAR1
Gene ID (NCBI) 54433
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9NY12
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GAR1, other named NOLA1, is a subunit of H/ACA and telomerase. Thought is not required for H/ACA protein assembly, GAR1 is necessary for ribosome biogenesis and telomere. H/ACA involves in class specify the sites of uridine-to-pseudouridine. It contains a core domain that flanked by glycine- and arginine-rich(GAR) domains. GAR1 also required for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transciptase(TERT) holoenzyme.

实验方案

Product Specific Protocols
WB protocol for GAR1 antibody 83577-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols