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验证数据展示

  • IF Staining of U2OS using 29847-1-AP

    IF Staining of U2OS using 29847-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using GARNL1 antibody (29847-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • WB analysis using 29847-1-AP

    WB analysis using 29847-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29847-1-AP (GARNL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

GARNL1 Polyclonal antibody

GARNL1 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 29847-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Synonyms

DKFZp566D133, DKFZp667F074, GARNL1, GRIPE, KIAA0884, p240, RALGAPA1, Tuberin like protein 1, TULIP1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, Jurkat cells
Positive IF/ICC detected inU2OS cells
Planning an IHC experiment? We recommend our IHCeasy GARNL1 Ready-To-Use IHC Kit. GARNL1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29847-1-AP targets GARNL1 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity Human
Immunogen GARNL1 fusion protein Ag31518 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name GTPase activating Rap/RanGAP domain-like 1
Synonyms DKFZp566D133, DKFZp667F074, GARNL1, GRIPE, KIAA0884, p240, RALGAPA1, Tuberin like protein 1, TULIP1
Calculated Molecular Weight230KD
Observed Molecular Weight250 kDa
GenBank Accession NumberNM_014990.3
Gene Symbol GARNL1
Gene ID (NCBI) 253959
RRIDAB_3086178
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6GYQ0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GARNL1/RalGAPα1, a major α subunit of the Ral-GTPase activating protein in skeletal muscle, as a protein whose phosphorylation and binding to the regulatory 14-3-3 proteins is stimulated by insulin and also by muscle contraction (PMID:24768767).The 100 kDa band maybe an isoform or fragment (BAA74907, 943aa).

实验方案

Product Specific Protocols
WB protocol for GARNL1 antibody 29847-1-APDownload protocol
IF protocol for GARNL1 antibody 29847-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols