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验证数据展示

  • IF Staining of U2OS using 29847-1-AP

    IF Staining of U2OS using 29847-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using GARNL1 antibody (29847-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • WB analysis using 29847-1-AP

    WB analysis using 29847-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29847-1-AP (GARNL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

GARNL1 Polyclonal antibody

GARNL1 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 29847-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

WB, IF/ICC, ELISA

别名

DKFZp566D133, DKFZp667F074, GARNL1, GRIPE, KIAA0884, p240, RALGAPA1, Tuberin like protein 1, TULIP1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, Jurkat cells
Positive IF/ICC detected inU2OS cells
Planning an IHC experiment? We recommend our IHCeasy GARNL1 Ready-To-Use IHC Kit. GARNL1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29847-1-AP targets GARNL1 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human
免疫原 GARNL1 fusion protein Ag31518 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 GTPase activating Rap/RanGAP domain-like 1
别名 DKFZp566D133, DKFZp667F074, GARNL1, GRIPE, KIAA0884, p240, RALGAPA1, Tuberin like protein 1, TULIP1
计算分子量230KD
观测分子量250 kDa
GenBank蛋白编号NM_014990.3
基因名称 GARNL1
Gene ID (NCBI) 253959
RRIDAB_3086178
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6GYQ0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GARNL1/RalGAPα1, a major α subunit of the Ral-GTPase activating protein in skeletal muscle, as a protein whose phosphorylation and binding to the regulatory 14-3-3 proteins is stimulated by insulin and also by muscle contraction (PMID:24768767).The 100 kDa band maybe an isoform or fragment (BAA74907, 943aa).

实验方案

Product Specific Protocols
WB protocol for GARNL1 antibody 29847-1-APDownload protocol
IF protocol for GARNL1 antibody 29847-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols