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RAP1GAP2 Polyclonal antibody

RAP1GAP2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25391-1-AP

Print datasheet

Synonyms

GARNL4, RAP1GA3, RAP1GA2, Rap1 GTPase-activating protein 2, KIAA1039

验证数据展示

  • WB analysis using 25391-1-AP

    WB analysis using 25391-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25391-1-AP (GARNL4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 25391-1-AP

    IHC staining of human stomach cancer using 25391-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 25391-1-AP (RAP1GAP2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25391-1-AP targets RAP1GAP2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen RAP1GAP2 fusion protein Ag21840 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name GTPase activating Rap/RanGAP domain-like 4
Synonyms GARNL4, RAP1GA3, RAP1GA2, Rap1 GTPase-activating protein 2, KIAA1039
Calculated Molecular Weight 730 aa, 80 kDa
Observed Molecular Weight80 kDa
GenBank Accession NumberBC140870
Gene Symbol RAP1GAP2
Gene ID (NCBI) 23108
RRIDAB_2880057
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ684P5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RAP1GAP2 antibody 25391-1-APDownload protocol
IHC protocol for RAP1GAP2 antibody 25391-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols