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验证数据展示

  • WB analysis using 67939-1-Ig

    WB analysis using 67939-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67939-1-Ig (GART antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis using 67939-1-Ig

    WB analysis using 67939-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67939-1-Ig (GART antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 67939-1-Ig

    IF Staining of HeLa using 67939-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using GART antibody (67939-1-Ig, Clone: 2A11E2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

GART Monoclonal antibody

GART Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67939-1-Ig

产品说明书

CloneNo. 2A11E2

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

AIR synthase, AIRS, GAR transformylase, GARS, GART, GARTF, PAIS, PGFT, PRGS

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HSC-T6 cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, PC-12 cells, NIH/3T3 cells, 4T1 cells
Positive IF/ICC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67939-1-Ig targets GART in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原 GART fusion protein Ag30509 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase
别名 AIR synthase, AIRS, GAR transformylase, GARS, GART, GARTF, PAIS, PGFT, PRGS
计算分子量 1010 aa, 108 kDa
观测分子量 46 kDa, 108 kDa
GenBank蛋白编号BC038958
基因名称 GART
Gene ID (NCBI) 2618
RRIDAB_2918691
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP22102
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GART antibody 67939-1-IgDownload protocol
IF protocol for GART antibody 67939-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols