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GART Monoclonal antibody

GART Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2A11E2

Cat no : 67939-1-Ig

Print datasheet

Synonyms

AIR synthase, AIRS, GAR transformylase, GARS, GART, GARTF, PAIS, PGFT, PRGS

验证数据展示

  • WB analysis using 67939-1-Ig

    WB analysis using 67939-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67939-1-Ig (GART antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis using 67939-1-Ig

    WB analysis using 67939-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67939-1-Ig (GART antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 67939-1-Ig

    IF Staining of HeLa using 67939-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using GART antibody (67939-1-Ig, Clone: 2A11E2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, HSC-T6 cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, PC-12 cells, NIH/3T3 cells, 4T1 cells
Positive IF/ICC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67939-1-Ig targets GART in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen GART fusion protein Ag30509 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase
Synonyms AIR synthase, AIRS, GAR transformylase, GARS, GART, GARTF, PAIS, PGFT, PRGS
Calculated Molecular Weight 1010 aa, 108 kDa
Observed Molecular Weight 46 kDa, 108 kDa
GenBank Accession NumberBC038958
Gene Symbol GART
Gene ID (NCBI) 2618
RRIDAB_2918691
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP22102
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GART antibody 67939-1-IgDownload protocol
IF protocol for GART antibody 67939-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols