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GAS1 Monoclonal antibody

GAS1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Pig

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2H8E1

Cat no : 67181-1-Ig

Print datasheet

Synonyms

GAS 1, GAS1, growth arrest 1

验证数据展示

  • WB analysis using 67181-1-Ig

    WB analysis using 67181-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67181-1-Ig (GAS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human gliomas using 67181-1-Ig

    IHC staining of human gliomas using 67181-1-Ig

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 67181-1-Ig (GAS1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human gliomas using 67181-1-Ig

    IHC staining of human gliomas using 67181-1-Ig

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 67181-1-Ig (GAS1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHEK-293 cells, SH-SY5Y cells, pig brain tissue, Y79 cells
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67181-1-Ig targets GAS1 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse, Pig samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human, Mouse, Pig
Immunogen GAS1 fusion protein Ag12259 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name growth arrest-specific 1
Synonyms GAS 1, GAS1, growth arrest 1
Calculated Molecular Weight 345 aa, 36 kDa
Observed Molecular Weight 36 kDa
GenBank Accession NumberBC074908
Gene Symbol GAS1
Gene ID (NCBI) 2619
RRIDAB_2882477
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP54826
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

GAS1 is involved in growth suppression. It is an integral plasma membrane protein whose expression is linked to growth arrest. It can be used as a novel therapeutic candidate for gastric cancer.

实验方案

Product Specific Protocols
WB protocol for GAS1 antibody 67181-1-IgDownload protocol
IHC protocol for GAS1 antibody 67181-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols