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验证数据展示

  • WB analysis of NIH/3T3 using 10072-1-AP

    WB analysis of NIH/3T3 using 10072-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10072-1-AP (GAS7 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 10072-1-AP

    IF Staining of HeLa using 10072-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GAS7 antibody (10072-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

GAS7 Polyclonal antibody

GAS7 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 10072-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (1)

应用

WB, IF/ICC, ELISA and More (1)

别名

KIAA0394, Growth arrest-specific protein 7, GAS-7, GAS 7

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inNIH/3T3 cells, mouse testis tissue
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10072-1-AP targets GAS7 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse
文献引用反应性human, mouse, rat
免疫原 GAS7 fusion protein Ag0114 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 growth arrest-specific 7
别名 KIAA0394, Growth arrest-specific protein 7, GAS-7, GAS 7
计算分子量 47 kDa
观测分子量 50 kDa
GenBank蛋白编号BC001152
基因名称 GAS7
Gene ID (NCBI) 8522
RRIDAB_2108090
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO60861
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Growth arrest-specific (GAS) genes are expressed preferentially in cells that enter a quiescent state. Gas7, first identified in serum-starved murine fibroblasts, encodes a 48-kDa protein. 3 isoforms of Gas7 were identified in rat brains, with molecular weight of 38, 47 and 55 kDa in SDS Page.

实验方案

Product Specific Protocols
WB protocol for GAS7 antibody 10072-1-APDownload protocol
IF protocol for GAS7 antibody 10072-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Biomater Sci

Dicalcium silicate microparticles modulate the differential expression of circRNAs and mRNAs in BMSCs and promote osteogenesis via circ_1983-miR-6931-Gas7 interaction.

Authors - Wenchao Zhong
View Article
ratWB,IHC

J Proteome Res

Comparative Proteomic Analysis of Primary Schwann Cells and a Spontaneously Immortalized Schwann Cell Line RSC 96: A Comprehensive Overview with a Focus on Cell Adhesion and Migration Related Proteins.

Authors - Ji Yuhua Y
View Article
humanWB

J Cancer

The METTL3/miR-196a Axis Predicts Poor Prognosis in Non-small Cell Lung Cancer

Authors - Zhen Yang
View Article