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GAT1 Polyclonal antibody

GAT1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse , rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20298-1-AP

Print datasheet

Synonyms

GABA Transporter 1, GABATHG, GABATR, GABT1, GAT 1, GAT1, SLC6A1

验证数据展示

  • WB analysis using 20298-1-AP

    WB analysis using 20298-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20298-1-AP (GAT1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse cerebellum using 20298-1-AP

    IHC staining of mouse cerebellum using 20298-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 20298-1-AP (GAT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inNeuro-2a cells, mouse testis tissue, rat testis tissue
Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20298-1-AP targets GAT1 in WB, IHC, ELISA applications and shows reactivity with human, mouse , rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse , rat
Cited Reactivitymouse
Immunogen GAT1 fusion protein Ag14109 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name solute carrier family 6 (neurotransmitter transporter, GABA), member 1
Synonyms GABA Transporter 1, GABATHG, GABATR, GABT1, GAT 1, GAT1, SLC6A1
Calculated Molecular Weight 67 kDa
Observed Molecular Weight 42 kDa, 67 kDa
GenBank Accession NumberBC033904
Gene Symbol GABA Transporter 1/GAT1
Gene ID (NCBI) 6529
RRIDAB_2878664
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP30531
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GAT1 antibody 20298-1-APDownload protocol
IHC protocol for GAT1 antibody 20298-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IHC

Chin Med

The effect of modified Qiyuan paste on mice with low immunity and sleep deprivation by regulating GABA nerve and immune system

Authors - Mei Rong
View Article