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  • Featured Product
  • KD/KO Validated

GATA1 Monoclonal antibody, PBS Only

GATA1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

5E2A8

Cat no : 60011-1-PBS

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Synonyms

ERYF1, Erythroid transcription factor, GATA 1, GATA binding factor 1, GATA1, GF 1, GF1, NF E1 DNA binding protein, NFE1, XLTT

验证数据展示

  • WB analysis using 60011-1-Ig (same clone as 60011-1-PBS)

    WB analysis using 60011-1-Ig (same clone as 60011-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60011-1-Ig (GATA1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60011-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast hyperplasia using 60011-1-Ig (same clone as 60011-1-PBS)

    IHC staining of human breast hyperplasia using 60011-1-Ig (same clone as 60011-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 60011-1-Ig (GATA1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60011-1-PBS in a different storage buffer formulation.

产品信息

60011-1-PBS targets GATA1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen GATA1 fusion protein Ag1350 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name GATA binding protein 1 (globin transcription factor 1)
Synonyms ERYF1, Erythroid transcription factor, GATA 1, GATA binding factor 1, GATA1, GF 1, GF1, NF E1 DNA binding protein, NFE1, XLTT
Calculated Molecular Weight 43 kDa
Observed Molecular Weight 43 kDa
GenBank Accession NumberBC009797
Gene Symbol GATA1
Gene ID (NCBI) 2623
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP15976
Storage Buffer PBS only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GATA1 is first identified as a member of the GATA transcription factor family, whose members bind the consensus (WGATAR) binding motif [PMID:22937757]. GATA1, a zinc finger DNA-binding transcription factor, plays a critical role in the normal development of hematopoietic cell lineages. The protein contains an N-terminal region that confers transcriptional activity and a C-terminal domain that mediates binding to DNA and other factors [PMID: 8524811]. GATA-1 is also implicated in regulating the expression of the erythroid and megakaryocytic-specific genes [PMID:22937757].