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验证数据展示

  • WB analysis using 60011-1-Ig (same clone as 60011-1-PBS)

    WB analysis using 60011-1-Ig (same clone as 60011-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60011-1-Ig (GATA1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60011-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 60011-1-Ig (same clone as 60011-1-PBS)

    IHC staining of mouse testis using 60011-1-Ig (same clone as 60011-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 60011-1-Ig (GATA1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60011-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 60011-1-Ig (same clone as 60011-1-PBS)

    IF Staining of HepG2 using 60011-1-Ig (same clone as 60011-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using GATA1 antibody (60011-1-Ig, Clone: 5E2A8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 60011-1-PBS in a different storage buffer formulation.

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  • KD/KO验证

GATA1 Monoclonal antibody, PBS Only

GATA1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 60011-1-PBS

产品说明书

CloneNo. 5E2A8

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

5E2A8, ERYF1, GATA-1, GATA-binding factor 1, GF1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

60011-1-PBS targets GATA1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag1350

Product name: Recombinant human GATA1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-316 aa of BC009797

Sequence: MEFPGLGSLGTSEPLPQFVDPALVSSTPESGVFFPSGPEGLDAAASSTAPSTATAAAAALAYYRDAEAYRHSPVFQVYPLLNCMEGIPGGSPYAGWAYGKTGLYPASTVCPTREDSPPQAVEDLDGKGSTSFLETLKTERLSPDLLTLGPALPSSLPVPNSAYGGPDFSSTFFSPTGSPLNSAAYSSPKLRGTLPLPPCEARECVNCGATATPLWRRDRTGHYLCNACGLYHKMNGQNRPLIRPKKRLIVSKRAGTQCTNCQTTTTTLWRRNASGDPVCNACGLYYKLHHQHYCGGSAQLMRAQSMASRGGVVSFS

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 GATA binding protein 1 (globin transcription factor 1)
别名 5E2A8, ERYF1, GATA-1, GATA-binding factor 1, GF1
计算分子量 43 kDa
观测分子量 43 kDa
GenBank蛋白编号BC009797
基因名称 GATA1
Gene ID (NCBI) 2623
RRIDAB_2294437
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP15976
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GATA1 is first identified as a member of the GATA transcription factor family, whose members bind the consensus (WGATAR) binding motif [PMID:22937757]. GATA1, a zinc finger DNA-binding transcription factor, plays a critical role in the normal development of hematopoietic cell lineages. The protein contains an N-terminal region that confers transcriptional activity and a C-terminal domain that mediates binding to DNA and other factors [PMID: 8524811]. GATA-1 is also implicated in regulating the expression of the erythroid and megakaryocytic-specific genes [PMID:22937757].

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