验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HepG2 cells, mouse liver tissue, K-562 cells, mouse heart tissue, rat heart tissue, HEK-293 cells, rat liver tissue |
| Positive IHC detected in | human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | SH-SY5Y cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11103-1-AP targets GATA2 in WB, IHC, IF/ICC, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, ChIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | GATA2 fusion protein Ag1557 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | GATA binding protein 2 |
| 别名 | NFE1B, GATA-binding protein 2, GATA binding protein 2, Endothelial transcription factor GATA-2 |
| 观测分子量 | 50-60 kDa |
| GenBank蛋白编号 | BC018988 |
| 基因名称 | GATA2 |
| Gene ID (NCBI) | 2624 |
| ENSEMBL Gene ID | ENSG00000179348 |
| RRID | AB_10914503 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P23769 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
What is the molecular weight of GATA2? Is GATA2 post-translationally modified?
The molecular weight of GATA2 is 51 kDa. There are two known isoforms of GATA2 that use alternative first exons (PMID: 10731672). GATA2 can be phosphorylated, which regulates its activity (PMID: 7876160).
What is the subcellular localization of GATA2?
GATA2 acts as a transcription factor in the nucleus. However, it can also be found in the cytoplasm (PMID: 25163535) and its phosphorylation promotes nuclear localization (PMID: 15837948).
What is the tissue expression pattern of GATA2?
GATA2 was initially identified as a hematopoietic transcription factor regulating the differentiation of hematopoietic stem cells but is also present in early erythroid cells, mast cells, and megakaryocytes. Additionally, it is expressed in the central nervous system (PMID: 10357893).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GATA2 antibody 11103-1-AP | Download protocol |
| IHC protocol for GATA2 antibody 11103-1-AP | Download protocol |
| IF protocol for GATA2 antibody 11103-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
EMBO J Functional screen reveals essential roles of miR-27a/24 in differentiation of embryonic stem cells. | ||
BMC Med Downregulation of AC092894.1 promotes oxaliplatin resistance in colorectal cancer via the USP3/AR/RASGRP3 axis | ||
PLoS Genet The PU.1-Modulated MicroRNA-22 Is a Regulator of Monocyte/Macrophage Differentiation and Acute Myeloid Leukemia. | ||
Int J Mol Sci LncRNA H19-Derived miR-675-5p Accelerates the Invasion of Extravillous Trophoblast Cells by Inhibiting GATA2 and Subsequently Activating Matrix Metalloproteinases. | ||
Endocrinology PGE2 and Thrombin Induce Myofibroblast Transdifferentiation via Activin A and CTGF in Endometrial Stromal Cells. |
