验证数据展示
经过测试的应用
| Positive WB detected in | MCF-7 cells, T-47D cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 2 publications below |
| WB | See 24 publications below |
| ChIP | See 1 publications below |
产品信息
10417-1-AP targets GATA3 in WB, ChIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, ChIP |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | GATA3 fusion protein Ag0174 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | GATA binding protein 3 |
| 别名 | Trans-acting T-cell-specific transcription factor GATA-3, GATA-binding factor 3, GATA binding factor 3 |
| 计算分子量 | 443 aa, 48 kDa |
| 观测分子量 | 37 kDa and 48 kDa |
| GenBank蛋白编号 | BC003070 |
| 基因名称 | GATA3 |
| Gene ID (NCBI) | 2625 |
| RRID | AB_2263174 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P23771 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GATA3 is a transcriptional factor that binds to the enhancer elements of all 4 T-cell antigen receptor genes. GATA3 is highly expressed in naive, freshly activated cells and Th2 lineage cells and thought to be necessary and sufficient for Th2 cytokine gene expression. GATA3 and TBET interfer with each other to regulate gene expression. This is a rabbit polyclonal antibody raised against part of C-terminus of human GATA3.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GATA3 antibody 10417-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cancer Cell Lunatic fringe deficiency cooperates with the Met/Caveolin gene amplicon to induce basal-like breast cancer. | ||
Cancer Res Cooperation between Pik3ca and p53 mutations in mouse mammary tumor formation. | ||
Oxid Med Cell Longev Androgen Plays a Potential Novel Hormonal Therapeutic Role in Th17 Cells Predominant Neutrophilic Severe Asthma by Attenuating BECs Regulated Th17 Cells Differentiation via MBD2 Expression | ||
Cell Death Dis Calreticulin promotes EGF-induced EMT in pancreatic cancer cells via Integrin/EGFR-ERK/MAPK signaling pathway. | ||
Development The inhibitor of histone acetyltransferases Nir regulates epidermis development. | ||
Int Immunopharmacol Modulating Th1/Th2 drift in asthma-related immune inflammation by enhancing bone mesenchymal stem cell homing through targeted inhibition of the Notch1/Jagged1 signaling pathway |
