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GATA3 Monoclonal antibody, PBS Only

GATA3 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B11A8

Cat no : 66400-1-PBS

Print datasheet

Synonyms

Trans-acting T-cell-specific transcription factor GATA-3, GATA-binding factor 3, GATA binding factor 3, 1B11A8

验证数据展示

  • WB analysis of MCF-7 using 66400-1-Ig (same clone as 66400-1-PBS)

    WB analysis of MCF-7 using 66400-1-Ig (same clone as 66400-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 66400-1-Ig (GATA3 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66400-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66400-1-Ig (same clone as 66400-1-PBS)

    IHC staining of human breast cancer using 66400-1-Ig (same clone as 66400-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66400-1-Ig (GATA3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66400-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 66400-1-Ig (same clone as 66400-1-PBS)

    IHC staining of human colon cancer using 66400-1-Ig (same clone as 66400-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66400-1-Ig (GATA3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66400-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 66400-1-Ig (same clone as 66400-1-PBS)

    IF Staining of MCF-7 using 66400-1-Ig (same clone as 66400-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using GATA3 antibody (66400-1-Ig, Clone: 1B11A8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66400-1-PBS in a different storage buffer formulation.

  • IF Staining of human breast cancer using 66400-1-Ig (same clone as 66400-1-PBS)

    IF Staining of human breast cancer using 66400-1-Ig (same clone as 66400-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 66400-1-Ig (GATA3 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66400-1-PBS in a different storage buffer formulation.

产品信息

66400-1-PBS targets GATA3 in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, IF-P, Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen GATA3 fusion protein Ag0174 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name GATA binding protein 3
Synonyms Trans-acting T-cell-specific transcription factor GATA-3, GATA-binding factor 3, GATA binding factor 3, 1B11A8
Calculated Molecular Weight 443 aa, 48 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC003070
Gene Symbol GATA3
Gene ID (NCBI) 2625
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP23771
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GATA3 is a transcriptional factor that binds to the enhancer elements of all 4 T-cell antigen receptor genes. GATA3 is highly expressed in naive, freshly activated cells and Th2 lineage cells and thought to be necessary and sufficient for Th2 cytokine gene expression. GATA3 and TBET interfer with each other to regulate gene expression.