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Species Reactivity
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验证数据展示

  • WB analysis of MCF-7 using 66400-1-Ig (same clone as 66400-1-PBS)

    WB analysis of MCF-7 using 66400-1-Ig (same clone as 66400-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 66400-1-Ig (GATA3 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66400-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66400-1-Ig (same clone as 66400-1-PBS)

    IHC staining of human breast cancer using 66400-1-Ig (same clone as 66400-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66400-1-Ig (GATA3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66400-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 66400-1-Ig (same clone as 66400-1-PBS)

    IHC staining of human colon cancer using 66400-1-Ig (same clone as 66400-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66400-1-Ig (GATA3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66400-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 66400-1-Ig (same clone as 66400-1-PBS)

    IF Staining of MCF-7 using 66400-1-Ig (same clone as 66400-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using GATA3 antibody (66400-1-Ig, Clone: 1B11A8 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66400-1-PBS in a different storage buffer formulation.

  • IF Staining of human breast cancer using 66400-1-Ig (same clone as 66400-1-PBS)

    IF Staining of human breast cancer using 66400-1-Ig (same clone as 66400-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 66400-1-Ig (GATA3 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66400-1-PBS in a different storage buffer formulation.

GATA3 Monoclonal antibody, PBS Only

GATA3 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA
Cat No. 66400-1-PBS

产品说明书

CloneNo. 1B11A8

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IF-P, Indirect ELISA

别名

Trans-acting T-cell-specific transcription factor GATA-3, GATA-binding factor 3, GATA binding factor 3, 1B11A8

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66400-1-PBS targets GATA3 in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 GATA3 fusion protein Ag0174 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 GATA binding protein 3
别名 Trans-acting T-cell-specific transcription factor GATA-3, GATA-binding factor 3, GATA binding factor 3, 1B11A8
计算分子量 443 aa, 48 kDa
观测分子量 50 kDa
GenBank蛋白编号BC003070
基因名称 GATA3
Gene ID (NCBI) 2625
RRIDAB_2881774
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP23771
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GATA3 is a transcriptional factor that binds to the enhancer elements of all 4 T-cell antigen receptor genes. GATA3 is highly expressed in naive, freshly activated cells and Th2 lineage cells and thought to be necessary and sufficient for Th2 cytokine gene expression. GATA3 and TBET interfer with each other to regulate gene expression.