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验证数据展示

  • WB analysis using 83808-1-RR (same clone as 83808-1-PBS)

    WB analysis using 83808-1-RR (same clone as 83808-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83808-1-RR (GATA3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83808-1-PBS in a different storage buffer formulation.

  • FC experiment of Jurkat using 83808-1-RR (same clone as 83808-1-PBS)

    FC experiment of Jurkat using 83808-1-RR (same clone as 83808-1-PBS)

    1x10^6 Jurkat cells were intracellularly stained with 0.25 ug GATA3 Recombinant antibody (83808-1-RR, Clone:240684A2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83808-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01933-1

    Sandwich ELISA standard curve of MP01933-1

    Sandwich ELISA standard curve of MP01933-1, Human GATA3 Recombinant Matched Antibody Pair - PBS only. 83808-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag0174. 83808-4-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL

  • Sample test of MP01933-1

    Sample test of MP01933-1

    The mean GATA3 concentration was determined to be 21.82 ng/mL in MCF-7 cell extract based on a 1.3 mg/mL extract load.

  • Cytometric bead array standard curve of MP01933-1

    Cytometric bead array standard curve of MP01933-1

    Cytometric bead array standard curve of MP01933-1, GATA3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83808-1-PBS. Detection antibody: 83808-4-PBS. Standard: Ag0174. Range: 0.781-100 ng/mL.

  • Affinity and Kinetic Characterization of 83808-1-RR

    Affinity and Kinetic Characterization of 83808-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83808-1-RR against Human GATA3 were performed. The affinity constant is 0.686 nM.

GATA3 Recombinant antibody, PBS Only (Capture)

GATA3 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 83808-1-PBS

产品说明书

CloneNo. 240684A2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

Trans-acting T-cell-specific transcription factor GATA-3, GATA-binding factor 3, GATA binding factor 3, 240684A2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83808-1-PBS targets GATA3 as part of a matched antibody pair:

MP01933-1: 83808-1-PBS capture and 83808-4-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human, mouse
免疫原 GATA3 fusion protein Ag0174 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 GATA binding protein 3
别名 Trans-acting T-cell-specific transcription factor GATA-3, GATA-binding factor 3, GATA binding factor 3, 240684A2
计算分子量 443 aa, 48 kDa
观测分子量 37 kDa and 48 kDa
GenBank蛋白编号BC003070
基因名称 GATA3
Gene ID (NCBI) 2625
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP23771
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Trans-acting T-cell-specific transcription factor, also known as GATA3, belongs to the zinc-finger transcription factor GATA family which drives the differentiation of T helper (Th) 2 cells. GATA factors are expressed in a tissue-specific manner and GATA3 is present in both hematopoietic (e.g., T cells) and non-hematopoietic tissues, including the kidney, central nervous system, skin, and mammary gland (PMID: 25148023). GATA3 plays a central role in Th2 responses both in vitro and in vivo (PMID: 16467870). The full-length GATA3 protein contains 444 aa corresponding to the molecular weights of 48 kDa, and the truncated protein was detected at approximately 37 kDa (PMID: 24758297).