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GATA3 Recombinant antibody

GATA3 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240684B8

Cat no : 83808-2-RR

Print datasheet

Synonyms

Trans-acting T-cell-specific transcription factor GATA-3, GATA-binding factor 3, GATA binding factor 3, 240684B8

验证数据展示

  • FC experiment of Jurkat using 83808-2-RR

    FC experiment of Jurkat using 83808-2-RR

    1x10^6 Jurkat cells were intracellularly stained with 0.25 ug GATA3 Recombinant antibody (83808-2-RR, Clone:240684B8) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • IF Staining of MCF-7 using 83808-2-RR

    IF Staining of MCF-7 using 83808-2-RR

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using GATA3 antibody (83808-2-RR, Clone: 240684B8 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

经过测试的应用

Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inJurkat cells, U-937 cells

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83808-2-RR is GATA3 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen GATA3 fusion protein Ag0174 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name GATA binding protein 3
Synonyms Trans-acting T-cell-specific transcription factor GATA-3, GATA-binding factor 3, GATA binding factor 3, 240684B8
Calculated Molecular Weight 443 aa, 48 kDa
GenBank Accession NumberBC003070
Gene Symbol GATA3
Gene ID (NCBI) 2625
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP23771
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GATA3 is a transcriptional factor that binds to the enhancer elements of all 4 T-cell antigen receptor genes. GATA3 is highly expressed in naive, freshly activated cells and Th2 lineage cells and thought to be necessary and sufficient for Th2 cytokine gene expression. GATA3 and TBET interfer with each other to regulate gene expression. This is a rabbit polyclonal antibody raised against part of C-terminus of human GATA3.

实验方案

Product Specific Protocols
IF protocol for GATA3 antibody 83808-2-RRDownload protocol
FC protocol for GATA3 antibody 83808-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols