• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

GATA4 Monoclonal antibody

GATA4 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2F5C6

Cat no : 68012-1-Ig

Print datasheet

Synonyms

GATA binding factor 4, GATA binding protein 4, GATA4, Transcription factor GATA 4

验证数据展示

  • WB analysis of PC-3 using 68012-1-Ig

    WB analysis of PC-3 using 68012-1-Ig

    PC-3 cells were subjected to SDS PAGE followed by western blot with 68012-1-Ig (GATA4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inPC-3 cells, HEK-293 cells, HepG2 cells, NCCIT cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68012-1-Ig targets GATA4 in WB, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, Mouse
Immunogen GATA4 fusion protein Ag30392 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name GATA binding protein 4
Synonyms GATA binding factor 4, GATA binding protein 4, GATA4, Transcription factor GATA 4
Calculated Molecular Weight 442 aa, 45 kDa
Observed Molecular Weight44,45 kDa
GenBank Accession NumberBC101580
Gene Symbol GATA4
Gene ID (NCBI) 2626
RRIDAB_2918759
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP43694
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GATA4 is a transcriptional activator. GATA4 binds to the consensus sequence 5'-AGATAG-3'. GATA4 acts as a transcriptional activator of ANF in cooperation with NKX2-5. Mutation of GATA4 will cause the atrial septal defect type 2 (ASD2).

实验方案

Product Specific Protocols
WB protocol for GATA4 antibody 68012-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols