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Species Reactivity
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Conjugate
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验证数据展示

  • WB analysis of PC-3 using 68012-1-Ig

    WB analysis of PC-3 using 68012-1-Ig

    PC-3 cells were subjected to SDS PAGE followed by western blot with 68012-1-Ig (GATA4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

GATA4 Monoclonal antibody

GATA4 Monoclonal Antibody for WB, ELISA
Cat No. 68012-1-Ig

产品说明书

CloneNo. 2F5C6

宿主/亚型

Mouse / IgG1

种属反应性

Human, Mouse

应用

WB, ELISA

别名

GATA binding factor 4, GATA binding protein 4, GATA4, Transcription factor GATA 4

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inPC-3 cells, HEK-293 cells, HepG2 cells, NCCIT cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68012-1-Ig targets GATA4 in WB, ELISA applications and shows reactivity with Human, Mouse samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human, Mouse
免疫原 GATA4 fusion protein Ag30392 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 GATA binding protein 4
别名 GATA binding factor 4, GATA binding protein 4, GATA4, Transcription factor GATA 4
计算分子量 442 aa, 45 kDa
观测分子量44,45 kDa
GenBank蛋白编号BC101580
基因名称 GATA4
Gene ID (NCBI) 2626
RRIDAB_2918759
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP43694
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GATA4 is a transcriptional activator. GATA4 binds to the consensus sequence 5'-AGATAG-3'. GATA4 acts as a transcriptional activator of ANF in cooperation with NKX2-5. Mutation of GATA4 will cause the atrial septal defect type 2 (ASD2).

实验方案

Product Specific Protocols
WB protocol for GATA4 antibody 68012-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols