验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, mouse liver tissue, mouse heart tissue, rat heart tissue, PC-3 cells, NIH/3T3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
19530-1-AP targets GATA4-Specific in WB, IHC, IF, IP, CoIP, ChiP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, CoIP, ChiP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, pig, monkey |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | GATA binding protein 4 |
| 别名 | GATA binding factor 4, GATA binding protein 4, GATA4, GATA4-Specific, Transcription factor GATA 4 |
| 计算分子量 | 45 kDa |
| 观测分子量 | 45 kDa |
| GenBank蛋白编号 | NM_002052 |
| 基因名称 | GATA4 |
| Gene ID (NCBI) | 2626 |
| RRID | AB_10642003 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P43694 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
What is the molecular weight of GATA4? Is GATA4 post-translationally modified?
The molecular weight of GATA4 is 48 kDa. GATA4 can be phosphorylated, which regulates its activity (PMID: 10788492).
What is the subcellular localization of GATA4?
GATA4 localizes to the nucleus. The nuclear localization motif is present in the C-terminal part of GATA4 (PMID: 9079680).
What is the tissue expression pattern of GATA4?
GATA4 was initially identified as a cardiac transcription factor important for the development of the heart and as one of the earliest transcription factors expressed in developing cardiac cells. However, GATA4 is expressed in numerous organs such as the liver, lung, gonads, and small intestine (PMID: 8455608).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GATA4-Specific antibody 19530-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Clin Transl Med Bmi-1-RING1B prevents GATA4-dependent senescence-associated pathological cardiac hypertrophy by promoting autophagic degradation of GATA4. | ||
Cardiovasc Res Circular RNA circRNA_000203 aggravates cardiac hypertrophy via suppressing miR26b-5p and miR-140-3p binding to Gata4. | ||
J Bone Miner Res Bmi-1 Overexpression Improves Sarcopenia Induced by 1,25(OH)2 D3 Deficiency and Downregulates GATA4-Dependent Rela Transcription | ||
Cell Prolif Carbon monoxide alleviates senescence in diabetic nephropathy by improving autophagy. |
