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GATA5 Polyclonal antibody

GATA5 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 55433-1-AP

Print datasheet

Synonyms

Transcription factor GATA-5, Transcription factor GATA 5, GATA-binding factor 5, GATA binding factor 5

验证数据展示

  • WB analysis of HepG2 using 55433-1-AP

    WB analysis of HepG2 using 55433-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 55433-1-AP (GATA5 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 55433-1-AP

    IHC staining of human colon using 55433-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 55433-1-AP (GATA5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 55433-1-AP

    IF Staining of HepG2 using 55433-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using GATA5 antibody (55433-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

经过测试的应用

Positive WB detected inHepG2 cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55433-1-AP targets GATA5 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IF, IP
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name GATA binding protein 5
Synonyms Transcription factor GATA-5, Transcription factor GATA 5, GATA-binding factor 5, GATA binding factor 5
Calculated Molecular Weight 41 kDa
Observed Molecular Weight 41 kDa
GenBank Accession NumberNM_080473
Gene Symbol GATA5
Gene ID (NCBI) 140628
RRIDAB_2881330
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BWX5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GATA5 antibody 55433-1-APDownload protocol
IHC protocol for GATA5 antibody 55433-1-APDownload protocol
IF protocol for GATA5 antibody 55433-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIP

Cancers (Basel)

Overexpression of GATA5 Inhibits Prostate Cancer Progression by Regulating PLAGL2 via the FAK/PI3K/AKT Pathway.

Authors - Qinghua Wang
View Article
human,mouseWB,IF,IP

Biochem Biophys Res Commun

Endothelial GATA5 positively regulates angiogenesis via cathepsin S-mediated Angpt2/Flk1 and MMP2/9 signaling pathways.

Authors - Anmin Ren
View Article