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GATA6 Polyclonal antibody

GATA6 Polyclonal Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (1)

Applications

WB, IHC, FC (Intra), ELISA and More (1)

Conjugate

Unconjugated

Cat no : 55435-1-AP

Print datasheet

Synonyms

Transcription factor GATA-6, Transcription factor GATA 6, GATA-binding factor 6

验证数据展示

  • WB analysis of MCF-7 using 55435-1-AP

    WB analysis of MCF-7 using 55435-1-AP

    WB result of GATA6 antibody (55435-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-GATA6 transfected MCF-7 cells.

  • WB analysis using 55435-1-AP

    WB analysis using 55435-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 55435-1-AP (GATA6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 55435-1-AP

    IHC staining of human colon cancer using 55435-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55435-1-AP (GATA6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 55435-1-AP

    IHC staining of human colon cancer using 55435-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 55435-1-AP (GATA6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat liver using 55435-1-AP

    IHC staining of rat liver using 55435-1-AP

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 55435-1-AP (GATA6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat liver using 55435-1-AP

    IHC staining of rat liver using 55435-1-AP

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 55435-1-AP (GATA6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of HuH-7 using 55435-1-AP

    FC experiment of HuH-7 using 55435-1-AP

    1X10^6 HuH-7 cells were intracellularly stained with 0.4 ug Anti-Human GATA6 (55435-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive WB detected inHeLa cells, BxPC-3 cells, MCF-7 cells
Positive IHC detected inhuman colon cancer tissue, rat liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHuH-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55435-1-AP targets GATA6 in WB, IHC, IF, FC (Intra), ELISA applications and shows reactivity with human, rat samples.

Tested Applications WB, IHC, FC (Intra), ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, rat
Cited Reactivityhuman, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name GATA binding protein 6
Synonyms Transcription factor GATA-6, Transcription factor GATA 6, GATA-binding factor 6
Calculated Molecular Weight 60 kDa
Observed Molecular Weight 60-64 kDa
GenBank Accession NumberNM_005257
Gene Symbol GATA6
Gene ID (NCBI) 2627
RRIDAB_11182815
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92908
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GATA6 is thought to be important for regulating terminal differentiation and/or proliferation.

实验方案

Product Specific Protocols
WB protocol for GATA6 antibody 55435-1-APDownload protocol
IHC protocol for GATA6 antibody 55435-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Environ Sci Technol

Perfluorinated Iodine Alkanes Promoted Neural Differentiation of mESCs by Targeting miRNA-34a-5p in Notch-Hes Signaling.

Authors - Zhihua Ren
View Article
mouseWB,IF

iScience

Commercial dishes with gelatin-free microstructured inserts for elongated stem cell self-renewal and pluripotency

Authors - Qian Ban
View Article
humanWB

FASEB J

Pulmonary expression of CYP2A13 and ABCB1 is regulated by FOXA2 and their genetic interaction is associated with lung cancer.

Authors - Chan Xiang
View Article
mouseWB

Front Cell Dev Biol

ADNP Controls Gene Expression Through Local Chromatin Architecture by Association With BRG1 and CHD4.

Authors - XiaoYun Sun
View Article
humanWB

Cell Rep

METTL14 regulates chromatin bivalent domains in mouse embryonic stem cells

Authors - Mandi Mu
View Article
humanWB

Hum Cell

MicroRNA-10a-5p-mediated downregulation of GATA6 inhibits tumor progression in ovarian cancer

Authors - Feiying Gao
View Article