验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, BxPC-3 cells, MCF-7 cells |
| Positive IHC detected in | human colon cancer tissue, rat liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC (Intra) detected in | HuH-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
55435-1-AP targets GATA6 in WB, IHC, IF, FC (Intra), ELISA applications and shows reactivity with human, rat samples.
| 经测试应用 | WB, IHC, FC (Intra), ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | GATA binding protein 6 |
| 别名 | Transcription factor GATA-6, Transcription factor GATA 6, GATA-binding factor 6 |
| 计算分子量 | 60 kDa |
| 观测分子量 | 60-64 kDa |
| GenBank蛋白编号 | NM_005257 |
| 基因名称 | GATA6 |
| Gene ID (NCBI) | 2627 |
| RRID | AB_11182815 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q92908 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GATA6 is a member of the GATA family of zinc-finger transcriptional regulators, which play crucial roles in the regulation of cell growth, differentiation, survival, and maintenance of body functions. Named after the conserved base sequence (G/A)GATA(A/T), GATA6 features conserved tandem zinc fingers and is essential in coordinating the development and precise gene regulation of diverse tissues, including the heart and gastrointestinal tract. GATA6 recognizes the sequence (A/T/C)GATA(A/T)A and interacts with other transcriptional regulators through its zinc-finger domain.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GATA6 antibody 55435-1-AP | Download protocol |
| IHC protocol for GATA6 antibody 55435-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
NPJ Precis Oncol CCL3 predicts exceptional response to TGFβ inhibition in basal-like pancreatic cancer enriched in LIF-producing macrophages | ||
Environ Sci Technol Perfluorinated Iodine Alkanes Promoted Neural Differentiation of mESCs by Targeting miRNA-34a-5p in Notch-Hes Signaling. | ||
iScience Commercial dishes with gelatin-free microstructured inserts for elongated stem cell self-renewal and pluripotency | ||
FASEB J Pulmonary expression of CYP2A13 and ABCB1 is regulated by FOXA2 and their genetic interaction is associated with lung cancer. | ||
Front Cell Dev Biol ADNP Controls Gene Expression Through Local Chromatin Architecture by Association With BRG1 and CHD4. | ||
