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GATAD2B Polyclonal antibody

GATAD2B Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 25679-1-AP

Print datasheet

Synonyms

Transcriptional repressor p66-beta, P66beta, p66/p68, GATA zinc finger domain-containing protein 2B

验证数据展示

  • WB analysis of A549 using 25679-1-AP

    WB analysis of A549 using 25679-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 25679-1-AP (GATAD2B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 25679-1-AP

    IF Staining of A431 using 25679-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using GATAD2B antibody (25679-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

经过测试的应用

Positive WB detected inA549 cells, HEK-293 cells, HeLa cells, K-562 cells, MCF-7 cells, PC-3 cells
Positive IF/ICC detected inA431 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25679-1-AP targets GATAD2B in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IP
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen GATAD2B fusion protein Ag22615 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name GATA zinc finger domain containing 2B
Synonyms Transcriptional repressor p66-beta, P66beta, p66/p68, GATA zinc finger domain-containing protein 2B
Observed Molecular Weight 69 kDa
GenBank Accession NumberBC069419
Gene Symbol GATAD2B
Gene ID (NCBI) 57459
RRIDAB_2880190
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WXI9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GATAD2B antibody 25679-1-APDownload protocol
IF protocol for GATAD2B antibody 25679-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Commun

Identification of proximal SUMO-dependent interactors using SUMO-ID.

Authors - Orhi Barroso-Gomila
View Article
mouseWB,IP

Cell Prolif

GATAD2B is required for pre-implantation embryonic development by regulating zygotic genome activation

Authors - Yuling Lin
View Article
humanWB

Nucleic Acids Res

CARM1 hypermethylates the NuRD chromatin remodeling complex to promote cell cycle gene expression and breast cancer development

Authors - Xue Chen
View Article